[Human rotavirus in cell culture: its isolation and passage]

Rotavirus was isolated from feces of patients and passaged in green monkey kidney cell subculture using the factors affecting the initiation of infection of tissue culture cells with human rotavirus. The effective factors were found to include the presence of low concentrations of trypsin in the infected culture and virus centrifugation of a cell layer which agreed with the data by Almeida et al. (1978) and Shoub and Bertran (1978) obtained in other cell systems. Immune electron microscopy was used to detect the virus in cell cultures and to prove its specificity.