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Real-time Monitoring of Ligand-receptor Interactions with Fluorescence Resonance Energy Transfer
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Published on: August 20, 2012

Sensitive fluorescence biosensor for folate receptor based on terminal protection of small-molecule-linked DNA

Xiaofeng Wei1, Wenlie Lin, Na Ma

  • 1Ministry of Education Key Laboratory of Analysis and Detection Technology for Food Safety (Fuzhou University), Department of Chemistry, Fuzhou University, Fuzhou, 350002, China.

Chemical Communications (Cambridge, England)
|May 17, 2012
PubMed

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Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...