Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 22, 2026

Longitudinal Evaluation of Mouse Hind Limb Bone Loss After Spinal Cord Injury using Novel, in vivo, Methodology
10:39

Longitudinal Evaluation of Mouse Hind Limb Bone Loss After Spinal Cord Injury using Novel, in vivo, Methodology

Published on: December 7, 2011

Multiple exposures to unloading decrease bone's responsivity but compound skeletal losses in C57BL/6 mice.

Shikha Gupta1, Surabhi Vijayaraghavan, Gunes Uzer

  • 1Integrative Skeletal Adaptation and Genetics Laboratory, Department of Biomedical Engineering, Stony Brook University, Stony Brook, NY 11794, USA.

American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
|May 18, 2012
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Innovative Technique for Single-Stage Phalloplasty with Complete Urethral Reconstruction for Micropenis and Perineal Hypospadias.

Indian journal of plastic surgery : official publication of the Association of Plastic Surgeons of India·2026
Same author

Maxillofacial tubercular osteomyelitis: diagnostic and therapeutic perspectives from a case series.

BMC infectious diseases·2026
Same author

Nutritional Composition, Therapeutic Benefits, and Functional Food Potential of <i>Coleus amboinicus</i>: A Comprehensive Review.

Food science & nutrition·2026
Same author

Internal jugular vein collapsibility index versus common carotid artery peak systolic velocity variation for prediction of post-spinal hypotension: A prospective observational study.

Journal of anaesthesiology, clinical pharmacology·2026
Same author

RACK1 and RPS6 as independent prognostic biomarkers in oral squamous cell carcinoma: a five-year survival analysis.

Frontiers in oral health·2026
Same author

Role of Artificial Intelligence in Pediatric Dentistry: An Umbrella Review.

International journal of paediatric dentistry·2026
Same journal

Changes in IMCL content and storage characteristics during an acute bout of high intensity interval exercise are influenced by sex and muscle fibre type.

American journal of physiology. Regulatory, integrative and comparative physiology·2026
Same journal

The Cardiorespiratory Response of Atlantic Salmon and Nile Tilapia to Similar Acute Thermal and Hypoxic Challenges: The Complete Story.

American journal of physiology. Regulatory, integrative and comparative physiology·2026
Same journal

Early Life Stress Blunts Time Of Day Specific Autonomic Function In Adult Male Mice.

American journal of physiology. Regulatory, integrative and comparative physiology·2026
Same journal

Homeostatic adaptations of the amphibious fish <i>Polypterus</i> in terrestrial environments.

American journal of physiology. Regulatory, integrative and comparative physiology·2026
Same journal

Renal Hemodynamic and Urinary Response to Baroreflex-Mediated Sympathetic Modulation: Insights from High-Resolution Arterial Impedance Analysis in Rats.

American journal of physiology. Regulatory, integrative and comparative physiology·2026
Same journal

Gravitational load, sex, and age-sex effects on cardiovascular compensatory responses during sit-to-stand and lie-to-stand transitions.

American journal of physiology. Regulatory, integrative and comparative physiology·2026
See all related articles

Multiple instances of hindlimb unloading (HLU) cause significant bone loss, but bone becomes less sensitive to mechanical unloading with repeated cycles. Recovery during reambulation is also impaired after subsequent HLU periods.

Area of Science:

  • Bone biology
  • Skeletal physiology
  • Mechanical unloading research

Background:

  • Mechanical unloading, such as hindlimb unloading (HLU), causes substantial bone loss.
  • The effects of repeated HLU and subsequent reambulation (RA) on bone health are not well understood.

Purpose of the Study:

  • To investigate the cumulative effects of multiple HLU/RA cycles on bone structure.
  • To determine if bone's response to mechanical unloading diminishes with repeated exposures.

Main Methods:

  • Adult male mice underwent one, two, or three cycles of 2-week HLU followed by 4-week RA.
  • In vivo micro-computed tomography (μCT) was used to longitudinally assess femur bone compartments.
  • Age-matched ambulatory mice served as controls.

More Related Videos

Modeling Highly Repetitive Low-level Blast Exposure in Mice
06:00

Modeling Highly Repetitive Low-level Blast Exposure in Mice

Published on: May 24, 2024

Proper Positioning and Restraint of a Rat Hind Limb for Focused High Resolution Imaging of Bone Micro-architecture Using In Vivo Micro-computed Tomography
04:24

Proper Positioning and Restraint of a Rat Hind Limb for Focused High Resolution Imaging of Bone Micro-architecture Using In Vivo Micro-computed Tomography

Published on: November 22, 2017

Related Experiment Videos

Last Updated: May 22, 2026

Longitudinal Evaluation of Mouse Hind Limb Bone Loss After Spinal Cord Injury using Novel, in vivo, Methodology
10:39

Longitudinal Evaluation of Mouse Hind Limb Bone Loss After Spinal Cord Injury using Novel, in vivo, Methodology

Published on: December 7, 2011

Modeling Highly Repetitive Low-level Blast Exposure in Mice
06:00

Modeling Highly Repetitive Low-level Blast Exposure in Mice

Published on: May 24, 2024

Proper Positioning and Restraint of a Rat Hind Limb for Focused High Resolution Imaging of Bone Micro-architecture Using In Vivo Micro-computed Tomography
04:24

Proper Positioning and Restraint of a Rat Hind Limb for Focused High Resolution Imaging of Bone Micro-architecture Using In Vivo Micro-computed Tomography

Published on: November 22, 2017

Main Results:

  • Multiple HLU/RA cycles exacerbated age-related bone loss compared to single exposure.
  • Cortical bone recovery was incomplete after each RA period, with significant reductions in 3x-HLU mice.
  • The magnitude of bone loss and recovery decreased with successive HLU/RA cycles, indicating reduced mechanosensitivity.

Conclusions:

  • Repeated mechanical unloading is more detrimental to bone than single episodes.
  • Bone exhibits reduced responsiveness to unloading and impaired recovery following consecutive HLU/RA cycles.
  • These findings highlight the importance of mechanical loading for maintaining skeletal integrity and suggest potential therapeutic targets for bone loss.