Related Experiment Video
Updated: May 22, 2026

Practical Aspects of Sample Preparation and Setup of 1H R1ρ Relaxation Dispersion Experiments of RNA
Published on: July 9, 2021
Rotamers or diastereomers? An overlooked NMR solution
Dennis X Hu1, Peter Grice, Steven V Ley
1Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW, United Kingdom.
Abstract:
The existence of rotamers in a solution of analyte complicates (1)H NMR analysis, especially when the presence of diastereomers is also possible. Organic chemists have often responded to this problem by conducting variable-temperature (VT) NMR experiments, changing NMR solvents, or adding complexing agents. Here, with specific examples, we illustrate the use of simple yet widely overlooked chemical-exchange NMR experiments which allow the nonintrusive rapid distinguishment of rapidly equilibrating small molecules such as rotamers from nonequilibrating diastereomers.
More Related Videos
Related Concept Videos
Stereoisomers
¹H NMR Chemical Shift Equivalence: Enantiotopic and Diastereotopic Protons
In chiral compounds such as 2-butanol, replacing the methylene hydrogens at C3 produces a pair of...
¹H NMR of Conformationally Flexible Molecules: Temporal Resolution
Racemic Mixtures and the Resolution of Enantiomers
Molecules with Multiple Chiral Centers
¹H NMR Chemical Shift Equivalence: Homotopic and Heterotopic Protons

