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Measuring the Densities of Aqueous Glasses at Cryogenic Temperatures
Published on: June 28, 2017
Supercooling and vitrification of aqueous glycerol solutions at normal and high pressures
K Miyata1, S Hayakawa, K Kajiwara
1Department of Applied Chemistry, National Defense Academy, Yokosuka 239-8686, Japan.
Abstract:
The supercooling and vitrification of aqueous glycerol solutions was studied at high pressures. Homogeneous ice nucleation temperatures (T(H)) were obtained for aqueous glycerol solutions of R=50, 30, 20, 12, and 10 (R: moles of water/moles of glycerol) up to 300MPa. The R=20 glycerol solution formed a glass above 200MPa at a cooling rate of 200°C/min, indicating that pressure enhances glass-formation of aqueous glycerol solutions. The (dT(g)/dP) values were obtained for vitrified aqueous glycerol solutions of R=3, 5, 10, and 20. These data can be used for the development of cryo-preservation liquids for living cells at high pressures.
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