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Updated: May 22, 2026

Isolation of Papillary and Reticular Fibroblasts from Human Skin by Fluorescence-activated Cell Sorting
Published on: May 7, 2019
Isolation and differentiation potential of fibroblast-like stromal cells derived from human skin
1Department of Medical Biotechnology and Laboratory Science and Research Center for Emerging Viral Infections, Chang Gung University, Tao-Yuan, Taiwan. hihuang@mail.cgu.edu.tw
Abstract:
Isolation of progenitor cell population is the first step in performing stem cell research. Progenitors isolated using different methods usually exhibit different characteristics and varied differentiation potentials. Here, we describe a method for isolation of the multipotent stromal cells from human foreskin tissues. The isolation rate is high and the obtained cells could be easily maintained in DMEM without supplement with specific growth factors.

