Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Human Platelet-Derived Extracellular Vesicles Are Internalized by Human Induced Pluripotent Stem Cell-Derived Neurons Under Control and Hypoxic Conditions.

Journal of extracellular biology·2026
Same author

Mechanical Loading Induces Distinct and Shared Responses in Endothelial and Muscle Cells and Reveals Exercise-Like Molecular Profiles.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2026
Same author

Human cardiac innervation-on-a-chip platform recapitulates neurocardiac interactions and supports future disease modeling.

Scientific reports·2026
Same author

Exploring the impact of human pluripotent stem cell heterogeneity on corneal limbal stem cell differentiation outcomes.

Scientific reports·2026
Same author

In Vivo Evaluation of Injected and Bioprinted Hyaluronic Acid-Based Bioink in Corneal Stromal Pocket.

Macromolecular bioscience·2026
Same author

In vitro identification of kainic acid-induced, concentration-dependent responses in human cortical neuronal networks.

Neuroscience·2025

Related Experiment Video

Updated: May 22, 2026

Cultivation of Human Neural Progenitor Cells in a 3-dimensional Self-assembling Peptide Hydrogel
11:01

Cultivation of Human Neural Progenitor Cells in a 3-dimensional Self-assembling Peptide Hydrogel

Published on: January 11, 2012

Three-dimensional growth matrix for human embryonic stem cell-derived neuronal cells.

Laura Ylä-Outinen1, Tiina Joki, Mari Varjola

  • 1NeuroGroup, Institute of Biomedical Technology, University of Tampere, Tampere, Finland; BioMediTech, Tampere, Finland; The Science Center of Pirkanmaa Hospital District, Tampere University Hospital, Tampere, Finland.

Journal of Tissue Engineering and Regenerative Medicine
|May 22, 2012
PubMed
Summary

PuraMatrix™ hydrogel supports human neural cell growth and electrical activity, making it a promising scaffold for neural tissue engineering applications.

Keywords:
PuraMatrixastrocytesencapsulationhydrogelneural tissue engineeringneuronal network activityoligodendrocytes

More Related Videos

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes
10:48

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes

Published on: April 12, 2015

Three-Dimensional Bioprinting of Human iPSC-Derived Neuron-Astrocyte Cocultures for Screening Applications
08:03

Three-Dimensional Bioprinting of Human iPSC-Derived Neuron-Astrocyte Cocultures for Screening Applications

Published on: September 29, 2023

Related Experiment Videos

Last Updated: May 22, 2026

Cultivation of Human Neural Progenitor Cells in a 3-dimensional Self-assembling Peptide Hydrogel
11:01

Cultivation of Human Neural Progenitor Cells in a 3-dimensional Self-assembling Peptide Hydrogel

Published on: January 11, 2012

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes
10:48

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes

Published on: April 12, 2015

Three-Dimensional Bioprinting of Human iPSC-Derived Neuron-Astrocyte Cocultures for Screening Applications
08:03

Three-Dimensional Bioprinting of Human iPSC-Derived Neuron-Astrocyte Cocultures for Screening Applications

Published on: September 29, 2023

Area of Science:

  • Biomaterials Science
  • Neuroscience
  • Stem Cell Biology

Background:

  • Tissue engineering of neuronal cells necessitates a 3D matrix that is soft, elastic, and supports cell proliferation.
  • Developing suitable biomaterials is crucial for advancing neural tissue regeneration and functional recovery.

Purpose of the Study:

  • To evaluate PuraMatrix™, a synthetic hydrogel, as a 3D growth matrix for human embryonic stem cell (hESC)-derived neural cells.
  • To assess the viability, maturation, migration, and electrical activity of neural cells within the PuraMatrix™ hydrogel.

Main Methods:

  • Culturing hESC-derived neural cells on, within, and under PuraMatrix™ hydrogels.
  • Monitoring cell viability and observing neural cell growth and process extension.
  • Characterizing neural network maturation and electrical activity using microelectrode arrays.

Main Results:

  • Human embryonic stem cell-derived neural cells exhibited good viability and growth on the PuraMatrix™ surface.
  • Neurons, astrocytes, and oligodendrocytes successfully grew, matured, and migrated within the hydrogel.
  • Electrically active neuronal networks were formed and confirmed via microelectrode array analysis.

Conclusions:

  • PuraMatrix™ serves as a supportive and effective 3D growth matrix for human neural cells.
  • This hydrogel holds potential as a key component for developing neuronal scaffolds in neural tissue engineering.