Related Experiment Video
Updated: May 22, 2026

10:28
Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
Published on: July 5, 2016
High-precision three-dimensional shape reconstruction via digital refocusing in multi-wavelength digital holography
Li Xu1, Carl C Aleksoff, Jun Ni
1Department of Mechanical Engineering, University of Michigan-Ann Arbor, Ann Arbor, Michigan 48109-2136, USA. xulium@umich.edu
Applied Optics
|May 23, 2012
Summary
This study introduces an extended-focus imaging (EFI) technique using digital refocusing and multiwavelength interferometry for precise 3D shape reconstruction. The holographic method overcomes depth-of-field limitations for accurate metrology measurements of out-of-focus objects.
Area of Science:
- Optical Metrology
- 3D Imaging
- Holography
Background:
- Depth-of-field constraints limit 3D shape reconstruction and metrology accuracy.
- Existing focus-detection methods struggle with high axial accuracy for out-of-focus 3D objects.
- Extended-focus imaging (EFI) offers potential for improved measurement range and precision.
Purpose of the Study:
- To present a holographic imaging solution for accurate 3D object measurement over a large depth range.
- To overcome limitations of classical depth-of-field constraints in 3D metrology.
- To enhance axial accuracy in profiling 3D objects using extended-focus imaging.
Main Methods:
- Incorporation of digital refocusing with multiwavelength interferometry.
- Development of a holographic imaging system for extended-focus imaging.
- Validation through digital simulations and refocusing experiments.
Main Results:
- The proposed EFI technique demonstrates accuracy and repeatability.
- Digital simulations and experiments confirm the method's effectiveness.
- High-precision 3D measurements of objects exceeding the classical depth of field were achieved.
Conclusions:
- The holographic EFI technique accurately measures 3D objects over extended depth ranges.
- This method overcomes significant depth-of-field limitations in 3D metrology.
- The approach enables high-precision 3D shape reconstruction for challenging objects.
Related Concept Videos
Three-Dimensional Microscopy in Microbiology
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
Confocal Fluorescence Microscopy
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

