Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

The Nucleolus

The Nucleolus

The nucleolus is the most prominent substructure of the nucleus. When it was first discovered, it was considered to be an isolated organelle that forms fibrils and granules. In 1931, the relationship between the nucleolus and chromosomes was first described by Heitz. He observed that the appearance and size of nucleolus varies depending on the stage of the cell cycle. He also noticed constricted regions on different chromosomes clustered together at definite cell cycle stages. These regions,...

Translation

Translation

Lesson: Translation
Translation is the process of synthesizing proteins from the genetic information carried by messenger RNA (mRNA). Following transcription, it constitutes the final step in the expression of genes. This process is carried out by ribosomes, complexes of protein and specialized RNA molecules. Ribosomes, transfer RNA (tRNA), and other proteins produce a chain of amino acids—the polypeptide—as the end product of translation.
Translation Produces the Building Blocks of Life

Translation

Translation

Lesson: Translation
Translation is the process of synthesizing proteins from the genetic information carried by messenger RNA (mRNA). Following transcription, it constitutes the final step in the expression of genes. This process is carried out by ribosomes, complexes of protein and specialized RNA molecules. Ribosomes, transfer RNA (tRNA), and other proteins produce a chain of amino acids—the polypeptide—as the end product of translation.
Translation Produces the Building Blocks of Life

Regulation of Nuclear Protein Sorting

Regulation of Nuclear Protein Sorting

Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

SenCat: Cataloging human cell senescence through multi-omic profiling of multiple senescent primary cell types.

Molecular cell·2026

Same author

Characterization of the m<sup>6</sup>A Epitranscriptome in Fibroblast Senescence.

Molecular and cellular biology·2026

Same author

Toward actionable interventions in human aging (12th ARDD meeting, 2025).

Aging·2026

Same author

SenCat: Cataloging human cell senescence through multiomic profiling of multiple senescent primary cell types.

bioRxiv : the preprint server for biology·2026

Same author

The Secretome of Human Trophoblast Stem Cells Attenuates Senescence-Associated Traits.

Aging cell·2026

Same author

Telomere attrition alters extracellular vesicles conferring adverse impacts on neuronal viability and inflammatory response.

iScience·2025

Search research articles

Related Experiment Video

Updated: May 22, 2026

Identification of Nucleolar Factors During HIV-1 Replication Through Rev Immunoprecipitation and Mass Spectrometry

Identification of Nucleolar Factors During HIV-1 Replication Through Rev Immunoprecipitation and Mass Spectrometry

Published on: June 26, 2019

RNA-binding protein nucleolin in disease.

Kotb Abdelmohsen¹, Myriam Gorospe

¹Laboratory of Molecular Biology and Immunology, National Institute on Aging-Intramural Research Program, National Institutes of Health, Baltimore, MD, USA. abdelmohsenk@mail.nih.gov

|May 24, 2012

Summary

Nucleolin protein regulates RNA and DNA metabolism, influencing cell proliferation and survival. Its diverse RNA-binding functions link it to diseases like cancer and viral infections, making it a therapeutic target.

More Related Videos

Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture

Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture

Published on: July 22, 2016

Related Experiment Videos

Last Updated: May 22, 2026

Identification of Nucleolar Factors During HIV-1 Replication Through Rev Immunoprecipitation and Mass Spectrometry

Identification of Nucleolar Factors During HIV-1 Replication Through Rev Immunoprecipitation and Mass Spectrometry

Published on: June 26, 2019

Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture

Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture

Published on: July 22, 2016

Area of Science:

Molecular Biology
Cell Biology
Biochemistry

Background:

Nucleolin is a key protein found in the nucleolus, nucleoplasm, cytoplasm, and cell membrane.
It plays critical roles in DNA metabolism and extensive RNA regulatory mechanisms.

Purpose of the Study:

To review the RNA-binding activities of nucleolin.
To explore its influence on gene expression and its impact on diseases.
To discuss the therapeutic potential of targeting nucleolin.

Main Methods:

Literature review of studies on nucleolin's function and disease association.
Analysis of nucleolin's RNA-binding domains and their role in post-transcriptional regulation.
Examination of nucleolin's involvement in cellular processes like proliferation and apoptosis.

Main Results:

Nucleolin binds to target RNAs via its RNA-binding and arginine/glycine-rich domains.
It modulates mRNA stability, translation, and microRNA processing.
Nucleolin influences cell proliferation and apoptosis, contributing to disease pathogenesis.

Conclusions:

Nucleolin's multifaceted RNA-binding activities are central to its role in various diseases, especially cancer and viral infections.
Targeting nucleolin presents a promising therapeutic strategy for these pathological conditions.