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Evaluation of Magainin I interactions with lipid membranes: an optical and electrochemical study
Jéssica M Nascimento1, Octávio L Franco, Maria D L Oliveira
1Programa de Pós-Graduação em Inovação Terapêutica, Universidade Federal de Pernambuco, 50670-901 Recife, PE, Brazil.
Abstract:
Most antimicrobial peptides (AMPs) have shown clear activity related to the disruption of lipid bilayers. In order to improve knowledge of this subject, the interaction of Magainin I (MagI) with phospholipid layers (PLs), uncoated or coated with synperonic (Synp), was studied using cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and surface plasmon resonance (SPR) techniques. MagI peptide was immobilized on gold electrode via a self-assembling monolayer obtained from liposomes and liposomes covered by Synp. MagI induces pores in the supported lipid membranes, which are reflected in an increased amperometric-response and also a decreased electron-transfer resistance (R(CT)). In addition, MagI showed a significant interaction with the PL-Synp-modified gold electrode, but MagI showed a reliable contact with the PL-modified gold electrode, leading to a decrease in the relative resistance charge transfer value of -17.38%. Our results demonstrated that Synp acts as a membrane sealant after exposure of the lipid membrane to MagI. A parallel reaction model was proposed for the interaction of MagI and a hybrid layer that result in a complex bimolecular interaction. In short, the importance of triblock copolymer to stabilize liposomes for future applications as drug delivery systems for MagI was demonstrated.
Insights
Antimicrobial peptides like Magainin I disrupt lipid bilayers. Synperonic acts as a sealant, stabilizing liposomes for potential drug delivery applications.
Area of Science:
- Biochemistry
- Biophysics
- Materials Science
Background:
- Antimicrobial peptides (AMPs) are known to disrupt lipid bilayers.
- Understanding the precise interaction mechanisms is crucial for developing new antimicrobial strategies and drug delivery systems.
Purpose of the Study:
- To investigate the interaction of Magainin I (MagI) with phospholipid layers (PLs), both uncoated and coated with synperonic (Synp).
- To explore the role of synperonic in modulating MagI's interaction with lipid membranes.
- To assess the potential of synperonic-coated liposomes as drug delivery systems for MagI.
Main Methods:
- Cyclic voltammetry (CV)
- Electrochemical impedance spectroscopy (EIS)
- Surface plasmon resonance (SPR)
- Immobilization of MagI peptide on gold electrodes via self-assembled monolayers from liposomes (uncoated and Synp-coated).
Main Results:
- MagI induced pore formation in supported lipid membranes, evidenced by increased amperometric response and decreased electron-transfer resistance (R(CT)).
- MagI showed significant interaction with PL-Synp-modified electrodes and reliable contact with PL-modified electrodes, reducing charge transfer resistance by -17.38%.
- Synperonic demonstrated a membrane-sealing effect after MagI exposure.
Conclusions:
- Synperonic acts as a membrane sealant, enhancing the stability of lipid membranes after interaction with MagI.
- A complex bimolecular interaction model was proposed for MagI with hybrid layers.
- Triblock copolymers like Synperonic are important for stabilizing liposomes, showing promise for MagI drug delivery applications.
