Real-time quantitative analysis of the influence of blue light on citrinin biosynthetic gene cluster expression in
Changlu Wang1, Hua Yang, Mianhua Chen
1Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Engineering and Biotechnology, Tianjin University of Science & Technology, No. 29, the 13th Avenue, TEDA, Tianjin 300457, People's Republic of China. changluwang2009@gmail.com
Abstract:
When Monascus MX was grown under blue light instead of in the dark, citrinin production increased from 478 mg l(-1) to 698 mg l(-1). To explain this, the expression of the pksCT gene, which encodes citrinin polyketide synthase, and of 5 ORFs around it, were monitored. Blue light enhanced citrinin production by upregulating the expression of orf1, orf3, and orf4, indicating that pksCT was not the key gene responsible for the quantity of citrinin production in blue light.
