Related Experiment Video
Updated: May 22, 2026

Characterizing Microbiome Dynamics – Flow Cytometry Based Workflows from Pure Cultures to Natural Communities
Published on: July 12, 2018
Flow cytometry in environmental microbiology: a rapid approach for the isolation of single cells for advanced
Belinda C Ferrari1, Tristrom J Winsley, Peter L Bergquist
1School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Randwick, NSW, Australia. b.ferrari@unsw.edu.au
Abstract:
The isolation and subsequent characterization of microbial cells from within environmental samples is a difficult process. Flow cytometry and cell sorting, when combined with the application of fluorescent probes, have the capability for the detection and separation of diverse microbial populations from within complex mixtures. The isolation of single cells allows for downstream investigations towards system-level characterization of unknown Bacterial Phyla to occur. We describe here the combination of fluorescent in situ hybridization and cell sorting for the detection and isolation of Candidate Division TM7 bacteria from an enriched soil sample. The result is the isolation of rare cells suitable for advanced molecular analysis including whole genome amplification and high-throughput pyrosequencing.
Related Concept Videos
Flow Cytometry
In...
Methods to Assess Microbial Populations
Overview Of Cell Separation And Isolation

