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Updated: May 22, 2026

Live Imaging of Mitosis in the Developing Mouse Embryonic Cortex

Live Imaging of Mitosis in the Developing Mouse Embryonic Cortex

Published on: June 4, 2014

Live imaging of the developing mouse mesonephros.

David Grote¹, Michael Marcotte, Maxime Bouchard

¹Goodman Cancer Research Centre and Department of Biochemistry, McGill University, Montreal, QC, Canada.

Methods in Molecular Biology (Clifton, N.J.)

|May 29, 2012

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This study presents a novel method for long-term live imaging of developing mouse embryos, overcoming challenges like phototoxicity. The technique allows detailed visualization of embryonic development, specifically the mesonephros, for extended periods.

Area of Science:

Developmental biology
Cellular and molecular imaging
Embryogenesis research

Background:

Embryonic development involves intricate tissue dynamics crucial for understanding morphogenesis.
Advanced imaging technologies enable in vivo visualization and 4D reconstruction of embryonic processes.
Challenges remain in maintaining embryo viability and minimizing phototoxicity during extended live imaging.

Purpose of the Study:

To describe a refined procedure for extended live imaging of intact mouse embryos.
To enable prolonged visualization of specific organ development, such as the mesonephros.
To provide a method adaptable for studying other embryonic structures.

Main Methods:

Development of a protocol for extended live imaging of mouse embryos.

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Analysis of Zebrafish Kidney Development with Time-lapse Imaging Using a Dissecting Microscope Equipped for Optical Sectioning

Analysis of Zebrafish Kidney Development with Time-lapse Imaging Using a Dissecting Microscope Equipped for Optical Sectioning

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Live Imaging of Mitosis in the Developing Mouse Embryonic Cortex

Live Imaging of Mitosis in the Developing Mouse Embryonic Cortex

Published on: June 4, 2014

In Situ Hybridization in Zebrafish Larvae and Juveniles during Mesonephros Development

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Analysis of Zebrafish Kidney Development with Time-lapse Imaging Using a Dissecting Microscope Equipped for Optical Sectioning

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Focus on imaging the developing mesonephros in vivo.

Utilizing intact embryo preparations to maintain physiological conditions.

Main Results:

Successful imaging of the developing mesonephros for up to 16 hours in intact mouse embryos.
Demonstration of a method to overcome limitations of embryo survival and phototoxicity in live imaging.
Establishment of a reproducible imaging procedure for embryonic development.

Conclusions:

The described method facilitates extended in vivo imaging of embryonic development, specifically the mesonephros.
This technique addresses critical challenges in embryo survival and phototoxicity for long-term live imaging.
The procedure is adaptable for studying the morphogenesis of various embryonic structures in mouse models.