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Updated: May 22, 2026

Analysis of Zebrafish Kidney Development with Time-lapse Imaging Using a Dissecting Microscope Equipped for Optical Sectioning

Analysis of Zebrafish Kidney Development with Time-lapse Imaging Using a Dissecting Microscope Equipped for Optical Sectioning

Published on: April 7, 2016

Live imaging kidney development in zebrafish.

Aleksandr Vasilyev¹, Iain A Drummond

¹Nephrology Division and Department of Pathology, Charlestown, MA, USA.

Methods in Molecular Biology (Clifton, N.J.)

|May 29, 2012

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Zebrafish embryos offer a transparent window into organ development. This study details a simple time-lapse microscopy method for observing zebrafish kidney and organ development, aiding vertebrate organ research.

Area of Science:

Developmental biology
Comparative anatomy
Regenerative medicine

Background:

The zebrafish (Danio rerio) is a valuable vertebrate model organism for studying organogenesis and regeneration.
Its transparent embryos allow for direct, non-invasive visualization of internal organ development.
Conserved nephron structure and function between zebrafish and humans facilitate translational research.

Purpose of the Study:

To present a straightforward time-lapse microscopy protocol for observing organ development in zebrafish.
To enable laboratories to investigate the intricate processes of kidney and other organ development in vivo.
To provide a tool for advancing the understanding of vertebrate organogenesis.

Main Methods:

Utilizing time-lapse microscopy for continuous observation of zebrafish embryos.

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Precise Cellular Ablation Approach for Modeling Acute Kidney Injury in Developing Zebrafish

Precise Cellular Ablation Approach for Modeling Acute Kidney Injury in Developing Zebrafish

Published on: June 3, 2017

In Situ Hybridization in Zebrafish Larvae and Juveniles during Mesonephros Development

In Situ Hybridization in Zebrafish Larvae and Juveniles during Mesonephros Development

Published on: August 13, 2021

Related Experiment Videos

Last Updated: May 22, 2026

Analysis of Zebrafish Kidney Development with Time-lapse Imaging Using a Dissecting Microscope Equipped for Optical Sectioning

Analysis of Zebrafish Kidney Development with Time-lapse Imaging Using a Dissecting Microscope Equipped for Optical Sectioning

Published on: April 7, 2016

Precise Cellular Ablation Approach for Modeling Acute Kidney Injury in Developing Zebrafish

Precise Cellular Ablation Approach for Modeling Acute Kidney Injury in Developing Zebrafish

Published on: June 3, 2017

In Situ Hybridization in Zebrafish Larvae and Juveniles during Mesonephros Development

In Situ Hybridization in Zebrafish Larvae and Juveniles during Mesonephros Development

Published on: August 13, 2021

Implementing a simple, reproducible procedure for laboratory settings.

Employing zebrafish as a model system due to its developmental transparency and genetic tractability.

Main Results:

Demonstration of a practical method for visualizing organ development in real-time.
Successful application of the technique to study zebrafish kidney development.
Establishment of a protocol that can be readily adopted by research laboratories.

Conclusions:

The described time-lapse microscopy procedure is an effective and accessible method for studying zebrafish organ development.
This technique enhances the utility of zebrafish as a model for understanding fundamental biological processes in organogenesis.
Findings from zebrafish organ development studies using this method have direct implications for human health and regenerative medicine.