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Updated: May 22, 2026
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Optical Clearing and Imaging of Immunolabeled Kidney Tissue
Published on: July 22, 2019
Immunohistochemical staining of dpERK staining during early kidney development
1Department of Genetics and Development, Columbia University, New York, NY, USA.
Methods in Molecular Biology (Clifton, N.J.)
|May 29, 2012
Abstract:
Signaling through the ERK/MAPK pathway within the Wolffian duct and ureteric bud epithelium is critical for kidney induction and branching morphogenesis. ERK signaling is activated by receptor tyrosine kinase such as RET and FGFR2. This protocol describes a method to detect the diphosphorylated form of ERK (dpERK) on paraffin embedded tissue of early mouse embryo.
