[Bacteriological diagnosis of tuberculosis]
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1UPMC université Paris-6, ER5, EA 1541, Laboratoire de bactériologie-hygiéne, F-75005 Paris. nicolas.veziris@upmc.fr
Abstract:
Today, microscopic examination and culture are still essential for active tuberculosis diagnosis and therapeutic decisions. Nucleic acid amplification and line probe assays speed up identification and susceptibility testing of mycobacteria in AFB smear positive specimens or in culture. They are also efficient for comparison of M. tuberculosis strains with each other (genotyping). On the other hand, today, molecular tests are not relevant for the diagnosis in smear negative specimens and even more for diagnosis of culture-negative tuberculosis.
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