Related Experiment Video
Updated: May 21, 2026

A Whole Cell Bioreporter Approach to Assess Transport and Bioavailability of Organic Contaminants in Water Unsaturated Systems
Published on: December 24, 2014
Biodegradation kinetics of phenanthrene by a fusant strain
Jing Lu1, Zhi Dang, Guining Lu
1College of Environmental Science and Engineering, South China University of Technology, Guangzhou Higher Education Mega Center, Guangzhou 510006, China. lujing19822@163.com
Abstract:
The fusant strain (F14), which produced by protoplast fusion between Sphingomonas sp. GY2B (GenBank DQ139343) and Pseudomonas sp. GP3A (GenBank EU233280), was tested for phenanthrene biodegradation at 30 °C and pH of 7.0. The kinetics of phenanthrene biodegradation by F14 was investigated over a wide range of initial concentration (15-1,000 mg l(-1)). The rate and the extent of phenanthrene degradation increased with the increase of concentration up to 230 mg l(-1), which indicated negligible inhibition effect at low concentrations. The non-competitive inhibition model was found to be fit for the process. GC-MS analysis showed that biodegradation of phenanthrene by F14 was via dioxygenation at both 1,2- and 3,4-positions and followed by 2-hydroxy-1-naphthoic acid and 1-hydroxy-2-naphthoic acid. The relative intensity of 2-hydroxy-1-naphthoic acid was approximately 3-4 times higher than that of 1-hydroxy-2-naphthoic acid, indicating the 2-hydroxy-1-naphthoic acid was the predominant product in the phenanthrene degradation by fusant strain F14.
More Related Videos
Related Concept Videos
Microbial Bioremediation of Pesticides
Microbial Bioremediation of Hydrocarbons
Microbial Bioremediation of Plastics
Bioplastics
Bioremediation
Biodeterioration

