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A Simple Protocol for Extracting Hemocytes from Wild Caterpillars
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Published on: November 15, 2012

[Functions of Calliphora vomitotia larval hemocytes in recognition and elimination from hemolymph human erythrocytes

T V Kind

    Tsitologiia
    |June 1, 2012
    PubMed

    Investigation of Calliphora vomitotia hemocyte defense reaction to human erythrocytes shows that erythrocytes are recognized mainly by thrombocytoids. Adhesion to plasmatocytes and subsequent phagocytosis also takes place, but in a less degree. Agglutination of erythrocytes by thrombocytoids is increased after feeding cessation and remains at a high level during all the period of crop emptying. Entrapped erythrocytes not later than after five to eight minutes show signs of destruction and disintegrate into fragments. Later structureless masses can arise. The results of secondary injections of charcoal particles reveal that both thrombocytoidal agglutinates and plasmatocytes can engulf additional abiotic invaders even after filling by erythrocytes. Meanwhile despite the changing morphology of agglutinates their capability to adhere new batches of aliens remains unchanged at enough high level. These agglutinates and plasmatocytes phagocytized the charcoal particles independently of previous erythrocyte exposition. Injection of charcoal and erythrocytes mixture leads to appearance of agglutinates with erythrocytes and charcoal mixed together. We guess that foreign receptors obtain wide spectrum of affinity to all kinds of invaders.

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