Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

Induced Pluripotent Stem Cells

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic cells are...

Cancer-Critical Genes I: Proto-oncogenes

Cancer-Critical Genes I: Proto-oncogenes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...

Cancer-Critical Genes I: Proto-oncogenes

Cancer-Critical Genes I: Proto-oncogenes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

SOPA and SIMPA: normalized single-sample integrated multiomics pathway analysis of tumor heterogeneity in solid cancers.

Briefings in bioinformatics·2026

Same author

Editorial: Honoring Dr. Angelo Azzi's legacy and expanding the horizons of Molecular Aspects of Medicine.

Molecular aspects of medicine·2026

Same author

β-Adrenergic Signaling Promotes Anti-Tumor Immunity in TP53-mutant Oral Squamous Cell Carcinoma.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

Same author

MicroRNAs in oncology: a translational perspective in the era of AI.

Nature reviews. Clinical oncology·2026

Same author

Transposable Element-Derived miR-28-5p and miR-708-5p: Exploring Potential Roles in Lung Cancer.

Non-coding RNA·2025

Same author

MicroRNAs as key regulators of cancer drug resistance: insights and future directions in chemotherapy, targeted-therapy, radiotherapy, and immunotherapy.

Cancer drug resistance (Alhambra, Calif.)·2025

Search research articles

Related Experiment Video

Updated: May 21, 2026

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

Published on: April 25, 2022

Cross talk between microRNA and coding cancer genes.

Tanja Kunej¹, Irena Godnic, Simon Horvat

¹Department of Animal Science, Biotechnical Faculty, University of Ljubljana, Domzale, Slovenia. tanja.kunej@bf.uni-lj.si

Cancer Journal (Sudbury, Mass.)

|June 1, 2012

Summary

MicroRNAs (miRNAs) regulate genes in human cancers. Aberrant miRNA expression drives cancer development, offering potential for new diagnostic markers and targeted therapies.

More Related Videos

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

Published on: October 7, 2025

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

Related Experiment Videos

Last Updated: May 21, 2026

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

Published on: April 25, 2022

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

Published on: October 7, 2025

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

Area of Science:

Molecular Biology
Genetics
Oncology

Background:

MicroRNAs (miRNAs) are noncoding RNAs that regulate gene expression post-transcriptionally.
Aberrant miRNA expression is implicated in the pathogenesis of all human cancers, acting as either tumor suppressors or oncogenes.
Complex regulatory networks involving miRNAs and protein-coding genes are crucial in cancer development.

Purpose of the Study:

To summarize key findings on miRNA-cancer associations.
To critically examine the intricate network connecting miRNAs and coding genes in cancer.
To identify potential diagnostic markers and therapeutic strategies for cancer.

Main Methods:

Literature review and data synthesis on miRNA-cancer interactions.
Development of a Web-based miRNA timeline tool (http://www.integratomics-time.com/miRNA_timeline) for data exploration.
Analysis of miRNA targeting mechanisms and subcellular functions.

Main Results:

miRNAs can inhibit or activate malignant potential by acting as tumor suppressors or oncogenes.
Differential miRNA expression in tumors is influenced by genomic location, epigenetics, and processing machinery malfunctions.
miRNAs interact with protein-coding genes through various mechanisms, affecting gene expression and cellular processes.

Conclusions:

Understanding miRNA-coding gene interactions is vital for developing novel cancer diagnostics and therapeutics.
The developed miRNA timeline tool aids researchers in navigating miRNA-cancer research and clinical applications.
Further research and tool development will elucidate complex ncRNA and coding gene interactions in disease.