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Related Experiment Video

Updated: May 21, 2026

Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform

Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform

Published on: November 10, 2016

A SERS-based immunoassay with highly increased sensitivity using gold/silver core-shell nanorods.

Lei Wu¹, Zhuyuan Wang, Shenfei Zong

¹Advanced Photonics Center, School of Electronic Science and Engineering, Southeast University, Nanjing 210096 Jiangsu, PR China.

Biosensors & Bioelectronics

|June 1, 2012

Summary

Researchers developed a highly sensitive immunoassay using gold/silver core-shell nanorods for surface-enhanced Raman scattering (SERS). This novel SERS probe significantly improves detection limits for biological sensing applications.

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Last Updated: May 21, 2026

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Area of Science:

Nanotechnology
Biomedical Engineering
Analytical Chemistry

Background:

Surface-enhanced Raman scattering (SERS) offers high sensitivity for molecular detection.
Gold nanorods are commonly used in SERS-based assays but have limitations in sensitivity.
Developing more sensitive SERS probes is crucial for advancing biological sensing.

Purpose of the Study:

To develop a highly sensitive immunoassay using novel gold/silver core-shell nanorods.
To characterize the properties and SERS activity of these core-shell nanorods.
To evaluate the performance of the developed immunoassay in terms of detection limit and sensitivity.

Main Methods:

Fabrication and characterization of gold/silver core-shell nanorods using UV-vis spectroscopy, TEM, EDX, and SERS.
Modification of nanorods with antibodies for immunoassay development.
Quantification of antigen concentration using SERS spectra and dose-response curves.

Main Results:

Gold/silver core-shell nanorods exhibited significantly higher SERS activity compared to uncoated gold nanorods.
The immunoassay demonstrated antigen concentration-dependent SERS spectra.
A detection limit as low as 70 fM was achieved, representing a 10,000-fold improvement over gold nanorod-based detection.

Conclusions:

Gold/silver core-shell nanorods are superior SERS probes for immunoassays due to enhanced sensitivity.
These nanorods offer a significant advancement over traditional gold nanorods in SERS-based detection.
The developed SERS probes show great potential for various biological sensing applications.