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Updated: May 21, 2026

In Vitro Culture of Epithelial Cells from Different Anatomical Regions of the Human Amniotic Membrane

In Vitro Culture of Epithelial Cells from Different Anatomical Regions of the Human Amniotic Membrane

Published on: November 28, 2019

Cell phenotypes in human amniotic fluid.

D A Davydova¹, E A Vorotelyak, Yu A Smirnova

¹Koltzov Institute of Developmental Biology, Russian Academy of Sciences;

|June 1, 2012

Summary

Amniotic fluid stem cells show potential for regenerative medicine. These cells proliferate long-term and exhibit broad differentiation markers, suggesting therapeutic utility without tumor formation.

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Area of Science:

Cell Biology
Regenerative Medicine
Developmental Biology

Background:

Stem cells are crucial for regenerative medicine.
Fetal stem cells, particularly from amniotic fluid (AF), are gaining attention.
AF stem cells offer a promising source for therapeutic applications.

Purpose of the Study:

To isolate and characterize stem cells from amniotic fluid (AF).
To evaluate the differentiation potential and safety of AF stem cells.
To explore the utility of AF stem cells in cell therapy.

Main Methods:

Isolation of stem cells from three AF samples.
Flow cytometry, RT-PCR, and immunohistochemistry for marker analysis.
In vivo transplantation studies in nude mice to assess tumor formation.

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Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions

Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions

Published on: November 27, 2017

Isolation, Cryopreservation and Culture of Human Amnion Epithelial Cells for Clinical Applications

Isolation, Cryopreservation and Culture of Human Amnion Epithelial Cells for Clinical Applications

Published on: December 21, 2014

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Last Updated: May 21, 2026

In Vitro Culture of Epithelial Cells from Different Anatomical Regions of the Human Amniotic Membrane

In Vitro Culture of Epithelial Cells from Different Anatomical Regions of the Human Amniotic Membrane

Published on: November 28, 2019

Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions

Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions

Published on: November 27, 2017

Isolation, Cryopreservation and Culture of Human Amnion Epithelial Cells for Clinical Applications

Isolation, Cryopreservation and Culture of Human Amnion Epithelial Cells for Clinical Applications

Published on: December 21, 2014

Main Results:

Isolated AF stem cells express mesenchymal, neural, epithelial, and pluripotency markers.
Cells demonstrated long-term proliferation in vitro.
Transplantation in nude mice showed no evidence of tumor formation.

Conclusions:

Putative stem/progenitor cells from AF possess broad differentiation potential.
AF stem cells are capable of long-term proliferation and appear safe for transplantation.
These cells hold significant promise for future cell therapy applications in regenerative medicine.