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Published on: September 10, 2014
[Establishment of a cisplatin-resistant human cervical cancer cell line]
Xin-Lian Chen1, He Wang, Xue-Mei Zhang
1Laboratory of Genetics, West China Institute of Women and Children's Health, West China Second Hospital, Sichuan University, Chengdu 610041, China.
Objective:
To establish a cisplatin (cDDP)-resistant human cervical cancer cell line named SiHa/cDDP and researched its biological characteristics.
Methods:
The development of cDDP resistance in SiHa cell line was induced by continuously stepwise exposure of the cells to cDDP. Cell growth curve, doubling time and resistance index (RI) were evaluated by MTT analysis. The expression of P-gp, GST-pi, Topo I were assessed with immunocytochemical method.
Results:
SiHa/cDDP cell line was successfully established which had stable growth, subculture, frozen reservation and resuscitation in the concentration of 2 microg/ml cDDP, doubling time was (45.82 +/- 3.69) h and RI to cDDP was 16.131. It also showed different degrees of cross-resistance to several anticancer drugs. As compared to parental SiHa, SiHa/cDDP had over-expression in P-gp and GST-pi (P < 0.01), but Topo I showed no statistically significant differences.
Conclusion:
We successfully established the cDDP-resistant human cervical cancer cell line SiHa/cDDP, which may provide ideal experimental model for research of human cervical carcinoma.
