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Related Concept Videos

Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
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Ribosomal RNA Synthesis

Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
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Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
Ribosomes01:27

Ribosomes

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Ribosomes01:27

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Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.
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Single Molecule Fluorescence Energy Transfer Study of Ribosome Protein Synthesis
08:07

Single Molecule Fluorescence Energy Transfer Study of Ribosome Protein Synthesis

Published on: July 6, 2021

Using Cross-links to Study Ribosomal Dynamics.

Valery I Ivanov1, Jason A Mears

  • 1a V.A. Engelhardt Institute of Molecular Biology Russian Academy of Sciences , 119991 , Moscow , Russia.

Journal of Biomolecular Structure & Dynamics
|June 2, 2012
PubMed
Summary
This summary is machine-generated.

This study reveals three distinct groups of ribosomal movements during protein synthesis, with some elements exhibiting large-scale mobility up to 95Å. Cross-linking data helps identify these dynamic structures and their roles in molecular switches.

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Area of Science:

  • Molecular Biology
  • Structural Biology
  • Biophysics

Background:

  • 3-D models of static ribosomes (large and small subunits) have been published.
  • Identifying movable ribosomal elements is crucial for understanding protein synthesis mechanics.

Purpose of the Study:

  • To characterize the dynamics of ribosomal elements during protein synthesis.
  • To apply cross-linking data analysis to study large-scale ribosomal movements.
  • To identify ribosomal components involved in molecular switches.

Main Methods:

  • Statistical analysis of cross-linking data.
  • Analysis of crystallographic and electron micrographic structures.
  • Quantification of ribosomal element motion magnitudes.

Main Results:

  • Three distinct groups of ribosomal motions were identified based on cross-linking data.
  • Group I: mean magnitude of 10Å.
  • Group II: most abundant, centered at 20Å with wide dispersion.
  • Group III: sparsely populated, with large distances up to 95Å, including the L7/12-stalk, L1-protuberance, and hairpins 88 and 89, indicating significant mobility.

Conclusions:

  • Cross-linking methods are effective for studying ribosomal dynamics, including large-scale functional movements.
  • Specific ribosomal elements, such as the L7/12-stalk and L1-protuberance, exhibit significant mobility.
  • This approach can help estimate the involvement of different structures in molecular switches during protein synthesis.