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Preparative enantioseparations using supercritical fluid chromatography.
1Amgen, 360 Binney Street, Cambridge, MA 02142, USA. miller@amgen.com
Journal of Chromatography. A
|June 5, 2012
Summary
Supercritical fluid chromatography (SFC) offers significant advantages for preparative enantioseparation in pharmaceutical research and development. This green technique enhances efficiency, reduces solvent use, and lowers purification costs compared to traditional methods.
Area of Science:
- Pharmaceutical R&D
- Analytical Chemistry
- Green Chemistry
Background:
- Preparative scale chromatographic enantioseparation is crucial for generating individual enantiomers in pharmaceutical R&D.
- Supercritical fluid chromatography (SFC) presents a viable alternative to traditional liquid chromatography for these separations.
Purpose of the Study:
- To highlight the advantages of supercritical fluid chromatography (SFC) over liquid chromatography for preparative enantioseparation.
- To emphasize SFC's role as a green and cost-effective separation technique.
Main Methods:
- Analytical scale screening of separation conditions.
- Preparative scale separations using SFC.
- Comparison of SFC with High-Performance Liquid Chromatography (HPLC).
Main Results:
- SFC enables rapid screening of separation conditions.
- SFC facilitates faster preparative separations and higher purification throughputs.
- SFC significantly reduces solvent consumption and waste generation compared to HPLC.
- Higher product concentrations are achievable post-SFC separation.
- SFC offers potential for reduced purification costs.
Conclusions:
- SFC is an advantageous technique for preparative enantioseparation in pharmaceutical R&D.
- SFC's green chemistry profile, characterized by reduced solvent usage, makes it an environmentally friendly option.
- The efficiency and cost-effectiveness of SFC support its routine application in drug development.
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