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Updated: May 21, 2026

Isolating, Sequencing and Analyzing Extracellular MicroRNAs from Human Mesenchymal Stem Cells

Isolating, Sequencing and Analyzing Extracellular MicroRNAs from Human Mesenchymal Stem Cells

Published on: March 8, 2019

Genome-scale analysis of human mRNA 5' coding sequences based on expressed sequence tag (EST) database.

Raffaella Casadei¹, Allison Piovesan, Lorenza Vitale

¹Center for Research in Molecular Genetics Fondazione CARISBO, Department of Histology, Embryology and Applied Biology, University of Bologna, via Belmeloro 8, 40126 Bologna, Italy.

|June 5, 2012

Summary

This study addresses the 5' end mRNA artifact by identifying extended coding regions in human mRNAs using an expressed sequence tag (EST)-based approach. Findings correct potential start codon misassignments, improving transcript annotation accuracy.

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Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis

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Published on: November 11, 2014

Area of Science:

Molecular Biology
Genomics
Bioinformatics

Background:

The 5' end mRNA artifact leads to incorrect start codon identification.
Accurate mRNA 5' end determination is crucial for understanding gene function.

Purpose of the Study:

To systematically identify extended coding regions at the 5' end of human mRNAs.
To address and correct the 5' end mRNA artifact in transcript annotation.

Main Methods:

Utilized an automated expressed sequence tag (EST)-based approach.
Analyzed over 7 million BLAT alignments of human mRNA sequences.
Performed in vitro cloning and sequencing for validation.

Main Results:

Identified extended 5' coding regions in 477 out of 18,665 human loci.
Confirmed findings for GNB2L1, QARS, and TDP2 cDNAs.
Generated a list of 20,775 human mRNAs with complete 5' coding sequences.

Conclusions:

The study provides a comprehensive correction for the 5' end mRNA artifact in human transcripts.
Improved transcript annotation enhances the accuracy of functional studies.
The identified extended regions and complete sequences refine our understanding of the human transcriptome.