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Updated: May 21, 2026

Ultrasound Assessment of Endothelial Function: A Technical Guideline of the Flow-mediated Dilation Test
Published on: April 27, 2016
Use of ultrasound for non-invasive assessment of flow-mediated dilation
Lee Stoner1, Manning J Sabatier
1School of Sport and Exercise, Massey University, Wellington, New Zealand. dr.l.stoner@gmail.com
Insights
Endothelial dysfunction, a precursor to atherosclerosis, can be assessed using the flow-mediated dilation (FMD) test. This non-invasive ultrasound technique helps predict cardiovascular disease events.
Area of Science:
- Cardiovascular Medicine
- Vascular Biology
- Diagnostic Imaging
Background:
- Atherosclerosis leads to heart attacks and strokes, major causes of death.
- Endothelial dysfunction precedes atherosclerosis development.
- Non-invasive assessment of endothelial function is clinically significant.
Purpose of the Study:
- To review the measurement of endothelial function using ultrasound.
- To focus on the flow-mediated dilation (FMD) technique for assessing endothelial function.
Main Methods:
- Utilizes ultrasound technology for vascular assessment.
- Employs the flow-mediated dilation (FMD) test as the primary method.
- Focuses on the standardized FMD technique.
Main Results:
- Reduced FMD is an early indicator of atherosclerosis.
- FMD capacity to predict future cardiovascular events is highlighted.
- Ultrasound-based FMD measurement is discussed.
Conclusions:
- Endothelial function assessment is crucial for cardiovascular health.
- The FMD test is a valuable tool for early atherosclerosis detection.
- Ultrasound provides a non-invasive method for evaluating endothelial function.
Abstract:
The pathological complications of atherosclerosis, namely heart attacks and strokes, remain the leading cause of mortality in the Western world. Preceding atherosclerosis is endothelial dysfunction. There is therefore interest in the application of non-invasive clinical tools to assess endothelial function. The flow-mediated dilation (FMD) test is the standard tool used to assess endothelial function. Reduced FMD is an early marker of atherosclerosis and has been noted for its capacity to predict future cardiovascular disease events. This review discusses the measurement of endothelial function using ultrasound, with a focus on the FMD technique.
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