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Related Experiment Video

Updated: May 21, 2026

Isolation, Culture, and Characterization of Dental Pulp Stem Cells from Human Deciduous and Permanent Teeth

Isolation, Culture, and Characterization of Dental Pulp Stem Cells from Human Deciduous and Permanent Teeth

Published on: May 17, 2024

Dentin regeneration using deciduous pulp stem/progenitor cells.

Y Zheng¹, X Y Wang, Y M Wang

¹Department of Endodontics, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Beijing, China.

Journal of Dental Research

|June 5, 2012

Summary

This summary is machine-generated.

This study shows that using porcine deciduous pulp stem/progenitor cells (PDPSCs) with a beta-tricalcium phosphate (β-TCP) scaffold can regenerate dentin. This approach offers a promising strategy for dental tissue repair and regeneration.

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Primary Culture of Dental Pulp Stem Cells

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Published on: May 17, 2024

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Published on: November 24, 2012

Primary Culture of Dental Pulp Stem Cells

Primary Culture of Dental Pulp Stem Cells

Published on: May 5, 2023

Area of Science:

Biomaterials Science
Regenerative Medicine
Dental Research

Background:

Reparative dentin formation is crucial for tooth integrity following injury or disease.
Stem/progenitor cell-based therapies are being explored for dental tissue regeneration.

Purpose of the Study:

To investigate stem/progenitor cell-based tissue engineering for dentin regeneration in a large animal model.
To determine the optimal cell density of porcine deciduous pulp stem/progenitor cells (PDPSCs) for dentin regeneration using a beta-tricalcium phosphate (β-TCP) scaffold.

Main Methods:

Porcine deciduous pulp stem/progenitor cells (PDPSCs) were cultured and combined with a β-TCP scaffold.
Different PDPSC concentrations were tested to assess cell attachment and alkaline phosphatase activity.
PDPSC/β-TCP constructs were used to repair pulp chamber roof defects in swine premolars, with cells labeled for tracking.

Main Results:

An optimal concentration of 5×10(5) PDPSCs/mL maximized cell attachment and alkaline phosphatase activity.
Transplanted PDPSCs were detected within the scaffold constructs after 4 weeks.
Significant regeneration of dentin-like structures and near-complete restoration of pulp chamber defects were observed at 16 weeks.

Conclusions:

PDPSC/β-TCP constructs demonstrate efficacy in direct pulp-capping procedures.
This study provides pre-clinical evidence supporting stem/progenitor cell-based strategies for dentin regeneration.
The findings highlight the potential of this approach for clinical applications in restorative dentistry.