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Updated: May 21, 2026
07:36
Isolation of Human Lymphatic Endothelial Cells by Multi-parameter Fluorescence-activated Cell Sorting
Published on: May 1, 2015
Increased lymphatic vessels in patients with encapsulating peritoneal sclerosis
Tatsuhiro Yaginuma1, Izumi Yamamoto, Hiroyasu Yamamoto
1Division of Kidney and Hypertension, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo, Japan. t-yaginuma@f00.itscom.net
Summary
Encapsulating peritoneal sclerosis (EPS) involves increased lymphatic vessels in the visceral peritoneum. This study clarifies lymphatic vessel morphology and proliferation in EPS patients, offering new insights into the disease's angiogenic response.
Area of Science:
- Vascular biology
- Peritoneal disease research
- Regenerative medicine
Background:
- Encapsulating peritoneal sclerosis (EPS) progression involves angiogenesis, but lymphatic vessel details in patients are unclear.
- Previous morphology studies were limited to the parietal peritoneum due to technical constraints.
- Analysis of both parietal and visceral lymphatic vessel morphology in EPS is needed.
Purpose of the Study:
- To analyze the morphology and angiogenic response of parietal and visceral lymphatic vessels in patients with encapsulating peritoneal sclerosis (EPS).
- To compare lymphatic vessel characteristics between EPS patients and controls.
- To investigate the role of lymphatic vessels in EPS progression.
Main Methods:
- Immunohistochemistry for CD34 (blood vessels), podoplanin (lymphatic vessels), and Ki-67 (proliferation) on peritoneal samples from 18 EPS patients and 17 controls.
- Immunogold electron microscopy for podoplanin localization.
- Comparison of parietal and visceral peritoneal membrane angiogenic responses in 7 EPS cases.
Main Results:
- Significantly increased CD34-positive (blood) and podoplanin-positive (lymphatic) capillaries per unit area in the visceral peritoneum of EPS patients compared to controls.
- Elevated proliferation marker Ki-67 in capillaries of EPS patients' visceral peritoneum.
- Visceral peritoneum showed a more prominent podoplanin-positive capillary profile than parietal peritoneum in EPS patients.
Conclusions:
- Lymphatic vessels are significantly increased in the visceral peritoneum of patients with encapsulating peritoneal sclerosis (EPS).
- The findings highlight the role of lymphatic vessel proliferation in the angiogenic response associated with EPS.
- This study provides crucial morphological data on lymphatic vessels in both parietal and visceral peritoneum in EPS.
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