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Related Experiment Video

Updated: May 21, 2026

Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization

Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization

Published on: August 5, 2008

Diversity arrays technology: a generic genome profiling technology on open platforms.

Andrzej Kilian¹, Peter Wenzl, Eric Huttner

¹Diversity Arrays Technology Pty Ltd, Yarralumla, Canberra, ACT, Australia. a.kilian@diversityarrays.com

Methods in Molecular Biology (Clifton, N.J.)

|June 6, 2012

Summary

Diversity Arrays Technology (DArT) offers a democratizing approach to DNA genotyping, leveraging genome complexity reduction and microarray analysis. This robust, cost-effective method accurately assesses DNA sequence variation across diverse species, including orphan crops.

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Area of Science:

Genomics and Bioinformatics
Molecular Biology
Plant Breeding and Genetics

Background:

Exponential growth in DNA sequence and polymorphism data over the past two decades.
Concentrated research investment on human and model species genomes.
Need for accessible, high-throughput genotyping technologies for a wider range of organisms.

Purpose of the Study:

To present Diversity Arrays Technology (DArT) as a democratizing genotyping solution.
To highlight DArT's robustness across different genome sizes and ploidy levels.
To detail DArT methods, protocols, and applications, including its evolutionary path.

Main Methods:

DArT utilizes genome complexity reduction methods to enrich for genic regions.
Assay readout is performed on open-access microarray platforms.
Quantitative nature of the assay allows for estimation of allelic frequencies.

Main Results:

DArT performance is driven by DNA sequence variation, not financial investment.
Demonstrated robustness across approximately 60 diverse organisms, including orphan crops.
Typical DArT assays test tens of thousands of genomic loci, reporting hundreds to thousands of markers.

Conclusions:

DArT provides a cost-effective and robust high-throughput genotyping technology.
Its simplicity and adaptability make it suitable for a wide range of species, including understudied ones.
The technology facilitates applications in population genetics and crop improvement through accurate polymorphism assessment.