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Mouse Round Spermatid Injection

Mouse Round Spermatid Injection

Published on: January 26, 2024

Technical note: Mice produced by intracytoplasmic sperm injection using a modified conventional method.

J Liu¹, G Y Lee, J A Lawitts

¹Center for Engineering in Medicine and Surgical Services, Massachusetts General Hospital, Harvard Medical School and Shriners Hospital for Children, Boston, MA 02114, USA. Liu.Jie@mgh.harvard.edu

Journal of Animal Science

|June 6, 2012

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This study presents a mercury-free method for mouse intracytoplasmic sperm injection (ICSI), achieving high efficiency and live births. The modified conventional technique avoids toxic mercury and complex equipment, making it a viable alternative.

Area of Science:

Reproductive Biology
Assisted Reproductive Technologies

Background:

Mercury-containing piezo-driven pipettes are standard for mouse intracytoplasmic sperm injection (ICSI).
Mercury's toxicity restricts its use in clinical settings, and mercury-free alternatives lack comparable efficiency.

Purpose of the Study:

To develop and validate a mercury-free, efficient method for mouse ICSI.
To establish a simplified, cost-effective alternative to existing ICSI techniques.

Main Methods:

A modified conventional method was employed, eliminating the need for mercury.
Oocyte survival, fertilization, and blastocyst development rates were assessed.
Embryo transfer to surrogate mothers was performed to evaluate live birth rates.

Main Results:

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Oocyte survival rate was 61.1% (77/126).
Fertilization rate (2-cell embryos) was 84.4% (65/77).
Blastocyst rate was 69.2% (45/65), and 11 live pups were born from 32 transferred embryos.

Conclusions:

The modified conventional method effectively eliminates mercury toxicity while maintaining high ICSI efficiency in mice.
This technique offers a simple, efficient, and mercury-free alternative for mouse ICSI, not requiring piezo-driven devices.