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Updated: May 21, 2026
Ratiometric Imaging of Extracellular pH in Dental Biofilms
Published on: March 9, 2016
Dental plaque pH variation with regular soft drink, diet soft drink and high energy drink: an in vivo study
Bhushan Arun Jawale1, Vikas Bendgude, Amit V Mahuli
1Department of Orthodontics and Dentofacial Orthopedics, Sinhgad Dental College and Hospital, Vadgaon, Maharashtra, India. bhushanjawale1@gmail.com
Background:
A high incidence of dental caries and dental erosion associated with frequent consumption of soft drinks has been reported. The purpose of this study was to evaluate the pH response of dental plaque to a regular, diet and high energy drink.
Methodology:
Twenty subjects were recruited for this study. All subjects were between the ages of 20 and 25 and had at least four restored tooth surfaces present. The subjects were asked to refrain from brushing for 48 hours prior to the study. At baseline, plaque pH was measured from four separate locations using harvesting method. Subjects were asked to swish with 15 ml of the respective soft drink for 1 minute. Plaque pH was measured at the four designated tooth sites at 5, 10 and 20 minutes intervals. Subjects then repeated the experiment using the other two soft drinks.
Results:
pH was minimum for regular soft drink (2.65 ± 0.026) followed by high energy drink (3.39 ± 0.026) and diet soft drink (3.78 ± 0.006). The maximum drop in plaque pH was seen with regular soft drink followed by high energy drink and diet soft drink.
Conclusion:
Regular soft drink possesses a greater acid challenge potential on enamel than diet and high energy soft drinks. However, in this clinical trial, the pH associated with either soft drink did not reach the critical pH which is expected for enamel demineralization and dissolution.
