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Immunolocalization of bone morphogenetic protein 2 during the early healing events after guided bone regeneration
Andréa Carvalho De Marco1, Maria Aparecida Neves Jardini, Filipe Modolo
1Department of Stomatology, University of São Paulo, São Paulo, Brazil. andreacdemarco@gmail.com
Objective:
The objective of this study was to evaluate the immunolocalization of bone morphogenetic protein 2 (BMP-2) after autogenous block grafting covered or not with an e-PTFE membrane.
Study Design:
Forty-eight rats were divided into 2 groups, autogenous block graft (B) and autogenous block graft + e-PTFE membrane (MB), and were evaluated by immunohistochemistry at baseline and 3, 7, 14, 21, and 45 days.
Results:
The largest number of positive cells in the recipient bed was observed after 3 days in both groups. At the graft border, the largest number of positive cells was seen after 7 days in group B and after 14 days in group MB. The highest proportion of staining in the graft was observed after 3 days in group B and after 21 days in group MB.
Conclusions:
High proportions of stain were related to intense revascularization and osteogenesis. Except for the interface, BMP-2 staining occurred later in group MB than in group B in all structures analyzed.
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