Related Experiment Video

Updated: Jan 20, 2026

Electrochemiluminescence Assays for Human Islet Autoantibodies
09:15

Electrochemiluminescence Assays for Human Islet Autoantibodies

Published on: March 23, 2018

16.0K

Development of an ultra-sensitive enzyme immunoassay for human insulin autoantibodies

Satoshi Numata1, Asako Umehara, Hideki Katakami

  • 1Department of Health Science, University of Kochi, Kochi 781-8515, Japan.

Clinical Biochemistry
|June 7, 2012
PubMed

More Related Videos

Developing a Salivary Antibody Multiplex Immunoassay to Measure Human Exposure to Environmental Pathogens09:08

Developing a Salivary Antibody Multiplex Immunoassay to Measure Human Exposure to Environmental Pathogens

Published on: September 12, 2016

9.1K
Detection of Human Islet Autoantibodies Using an Electrochemiluminescence Assay06:09

Detection of Human Islet Autoantibodies Using an Electrochemiluminescence Assay

524

Related Concept Videos

Electrochemiluminescence Assays for Human Islet Autoantibodies09:15

Electrochemiluminescence Assays for Human Islet Autoantibodies

Here, we presented the methods to detect human islet autoantibodies using electrochemiluminescence (ECL) assays. The protocol, used to predict type 1 diabetes, can be expanded upon to detect autoantibodies for other autoimmune...
16.0K
Developing a Salivary Antibody Multiplex Immunoassay to Measure Human Exposure to Environmental Pathogens09:08

Developing a Salivary Antibody Multiplex Immunoassay to Measure Human Exposure to Environmental Pathogens

In the current climate of scarce resources, new technologies are emerging that allow researchers to conduct studies cheaper, faster and with more precision. Here we describe the development of a bead-based salivary antibody multiplex immunoassay to measure human exposure to multiple environmental pathogens...
9.1K
Granulocyte-dependent Autoantibody-induced Skin Blistering12:23

Granulocyte-dependent Autoantibody-induced Skin Blistering

In the animal model described in our present work, purified IgG antibodies against a stretch of 200 amino acids (aa 757-967) of collagen VII are injected repeatedly into mice reproducing the blistering phenotype as well as the histo- and immunopathological features characteristic to human epidermolysis bullosa acquisita...
10.9K
Insulin Injection and Hemolymph Extraction to Measure Insulin Sensitivity in Adult Drosophila melanogaster05:52

Insulin Injection and Hemolymph Extraction to Measure Insulin Sensitivity in Adult Drosophila melanogaster

Conserved insulin signaling pathways found in the fruit fly Drosophila melanogaster make this organism a potential tool for modeling metabolic disorders including type II diabetes. To this end, it is critical to establish physiological assays to effectively measure systemic insulin action in peripheral glucose disposal in the adult...
20.3K
Detection of Human Islet Autoantibodies Using an Electrochemiluminescence Assay06:09

Detection of Human Islet Autoantibodies Using an Electrochemiluminescence Assay

This video showcases the electrochemiluminescence detection of islet autoantibodies in human serum. The antibodies in the serum bridge the ruthenium-conjugated antigen to the biotinylated capture antigen, forming complexes that are detected by electrochemiluminescence...
524
An Assay to Detect Autoantibodies in Human Serum using a Hippocampal Neuronal Culture03:52

An Assay to Detect Autoantibodies in Human Serum using a Hippocampal Neuronal Culture

This video demonstrates a technique for identifying antibodies against specific antigens in a sample using a rat hippocampal neuronal culture. By exposing cultured neurons to patient samples containing autoantibodies targeting N-Methyl-D-aspartate receptors (NMDARs), the presence of autoantibodies bound to the neuronal surface is revealed through...
467