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Related Experiment Video

Updated: May 21, 2026

Micro-dissection of Enamel Organ from Mandibular Incisor of Rats Exposed to Environmental Toxicants

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Published on: March 29, 2018

Cracks in dentin and enamel after cryopreservation.

Sebastian Kühl¹, Hans Deyhle, Melanie Zimmerli

¹School of Dental Medicine, Department of Oral Surgery, Oral Radiology and Oral Medicine, University of Basel, Basel, Switzerland. Sebastian.kuehl@unibas.ch

Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology

|June 7, 2012

Summary

Cryopreservation of teeth for long-term storage did not create significant cracks in dentin or cementum. However, forceps used during extraction can cause noticeable enamel cracks, impacting tooth integrity.

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Area of Science:

Dental Science
Biomaterials Science
Cryobiology

Background:

Long-term storage of teeth is crucial for various dental applications, including transplantation and research.
Understanding the effects of cryopreservation on tooth hard tissues is essential to ensure their structural integrity.

Purpose of the Study:

To investigate the potential for cryopreservation to induce cracks in tooth enamel and dentin.
To assess the structural integrity of teeth after long-term cryopreservation.

Main Methods:

Three extracted human teeth were cryopreserved in liquid nitrogen at -196°C for four months.
Micro computed tomography (micro-CT) utilizing synchrotron radiation was employed to detect and analyze cracks in tooth hard tissues.

Main Results:

No cracks wider than 0.8 μm were detected in the dentin or cementum of cryopreserved teeth.
Enamel cracks were observed in all teeth, but these were attributed to forceps application during the extraction process.

Conclusions:

Standard cryopreservation protocols for teeth do not appear to generate cracks exceeding 0.8 μm in dentin and cementum.
The mechanical forces from forceps during tooth extraction are a significant factor in causing visible enamel cracks, independent of cryopreservation.