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Safety assessment of Bacillus licheniformis Me1 isolated from milk for probiotic application

Vadakedath Nithya1, Serva P Muthukumar, Prakash M Halami

  • 1Department of Food Microbiology, CSIR-Central Food Technological Research Institute, Mysore, India.

International Journal of Toxicology
|June 8, 2012
PubMed

Insights

Bacillus licheniformis Me1, a milk isolate, underwent safety testing. Studies showed no adverse effects in rats, mice, or rabbits, indicating its safety for food industry use.

Area of Science:

  • Microbiology
  • Toxicology
  • Food Safety

Background:

  • Bacillus licheniformis is a common bacterium found in various environments.
  • Probiotics require rigorous safety assessments before food industry application.
  • Strain Me1 was isolated from milk, necessitating toxicological evaluation.

Purpose of the Study:

  • To conduct a comprehensive in vivo toxicological safety assessment of Bacillus licheniformis Me1.
  • To determine the No-Observed-Adverse-Effect Level (NOAEL) for Bacillus licheniformis Me1.
  • To evaluate the genotoxic and irritation potential of Bacillus licheniformis Me1.

Main Methods:

  • Acute and 90-day subchronic oral toxicity studies in Wistar rats.
  • Serum biochemical, hematological, and histopathological analyses.
  • In vivo micronucleus assay in mice and dermal/eye irritation tests in rabbits.

Main Results:

  • No treatment-related illness or mortality observed in acute or subchronic oral toxicity studies.
  • No significant differences in hematology, serum biochemistry, or histopathology compared to controls.
  • No genotoxic effects in mice and no dermal or ocular irritation in rabbits.

Conclusions:

  • The No-Observed-Adverse-Effect Level (NOAEL) for Bacillus licheniformis Me1 is greater than 1.1 × 10(11) colony-forming units/kg body weight.
  • Bacillus licheniformis Me1 demonstrated a lack of genotoxicity and local irritation.
  • Bacillus licheniformis Me1 is considered safe for applications in the food industry.

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