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Whole Genome Analysis of Limosilactobacillus fermentum MCC0552 for Probiotic Functionalities and Comparative Genomic
Krishna Komatwar1,2, Aravind Sundararaman1,3, Steji Raphel1,2
1Microbiology and Fermentation Technology Department, CSIR- CFTRI, Mysore, India, 570020.
Probiotics and Antimicrobial Proteins
|February 19, 2025
Summary
Limosilactobacillus fermentum MCC0552, a dairy-isolated probiotic, possesses significant genomic potential for vitamin synthesis and immune modulation. Its whole genome analysis reveals unique genetic features for enhanced probiotic applications.
Area of Science:
- Microbiology and Genomics
- Probiotic Research
- Nutraceutical Science
Background:
- Limosilactobacillus fermentum MCC0552 is a dairy-derived probiotic with established immuno-modulatory effects.
- Previous research indicates its ability to alleviate inflammatory and diabetic conditions.
- Understanding its genomic basis is crucial for optimizing its probiotic functions.
Purpose of the Study:
- To perform whole genome sequencing of L. fermentum MCC0552.
- To identify genes associated with probiotic functionalities.
- To compare its genome with reference strains for evolutionary insights.
Main Methods:
- Whole genome sequencing and analysis of L. fermentum MCC0552.
- Phylogenetic analysis using reference L. fermentum strains.
- Functional annotation for probiotic traits, including vitamin biosynthesis and mucin binding.
- Genomic island (GI) and prophage region identification.
Main Results:
- The genome of L. fermentum MCC0552 contains genes for vitamin biosynthesis (thiamine, riboflavin) and mucin binding proteins.
- HPLC confirmed synthesis of B vitamins (B1, B2, B6, B9, B12).
- Nineteen genomic islands (GIs) were identified, including CRISPR, TA systems, and heavy metal resistance genes.
- Two intact prophage regions were detected, contributing to probiotic attributes.
Conclusions:
- L. fermentum MCC0552 exhibits significant genomic potential for probiotic applications.
- The identified genetic features, including vitamin synthesis and immune-related genes, enhance its nutritional and nutraceutical value.
- This study provides a genomic foundation for leveraging L. fermentum MCC0552 in diverse scientific and commercial fields.
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