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Natural Product Discovery with LC-MS/MS Diagnostic Fragmentation Filtering: Application for Microcystin Analysis
Published on: May 31, 2019
Comparative proteomics between natural Microcystis isolates with a focus on microcystin synthesis
Angela Tonietto1, Bernardo A Petriz, Wérika C Araújo
1Centro de Análises Proteômicas e Bioquímicas, Universidade Católica de Brasília, Pós Graduação em Ciências Genômicas e Biotecnologia, SGAN 916 Norte Av, W5, Brasília, DF, Brazil. ocfranco@gmail.com.
Proteome Science
|June 9, 2012
Summary
Proteomics revealed that Microcystis aeruginosa strains producing microcystins have higher levels of metabolic enzymes. This suggests a link between energy metabolism and cyanotoxin synthesis in these common aquatic bacteria.
Area of Science:
- Environmental microbiology
- Proteomics
- Cyanobacteria research
Background:
- Microcystis aeruginosa is a globally prevalent cyanobacterium.
- It produces microcystin, a potent cyanotoxin linked to animal poisonings.
- Understanding microcystin synthesis in cyanobacteria remains limited.
Purpose of the Study:
- To compare microcystin production in two M. aeruginosa strains using proteomic tools.
- To investigate the protein expression differences between toxin-producing and non-toxin-producing strains.
- To identify proteins potentially involved in microcystin synthesis.
Main Methods:
- Two-dimensional gel electrophoresis (2-DE) was employed to separate proteins.
- Differential protein spots between strains were identified.
- Tandem mass spectrometry was used for protein identification.
Main Results:
- 30 differential protein spots were identified between the two M. aeruginosa strains.
- 57% of identified proteins were related to energy metabolism and upregulated in the toxin-producing strain.
- Specific proteins unique to each strain and differentially expressed proteins were noted.
Conclusions:
- Higher quantities of metabolic enzymes in toxin-producing strains may correlate with microcystin metabolism.
- Microcystin production might involve proteins beyond those directly in its synthesis pathway, including those in the Calvin cycle and glycolysis.
