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Updated: May 21, 2026
Methylated DNA Immunoprecipitation
Published on: January 2, 2009
[Development of a method for methylated DNA enrichment with functionalized mesocellular silica foams immobilized with
Ya-ting Chen1, Lu Han, Dong-yuan Zhao
1Key Laboratory of Molecular Medicine, Ministry of Education, Shanghai Medical College, Institute of Biomedical Science, Fudan University, Shanghai 200032, P. R. China.
Objective:
To develop a method for enriching methylated DNA in clinical samples using mesocellular silica foams (MCFs) immobilized with methyl-CpG binding domain (MBD).
Methods:
MCFs with ultra-large pore size were synthesized, functionalized and immobilized with GST-MBD.
Results:
The large cage-like pore structures of MCF materials was retained after functionalization and immobilization, with pore diameter of 55 nm, window size of 30 nm, and a high pore volume of 1.0 cm(3)/g. The loading amount of MBD was as high as 53 wt%. Immobilized MBD showed high binding activity and stability. In a binding buffer with salt concentrations ranging 500-550 mmol/L, the MCF-MBD can selectively enrich methylated DNA from the mixed DNA solution.
Conclusion:
The MCF-MBD method may offer a better choice for high-throughout DNA methylation screening, and has laid a foundation for clinical application, prenatal diagnosis and research on DNA methylation-related genetic diseases.
