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Published on: August 23, 2013
Histamine uptake by human normal neutrophils in vitro
1Dipartimento di Anatomia Umana e Istologia, Università di Firenze, Italy.
Basic and Applied Histochemistry
|January 1, 1990
Summary
Human neutrophils efficiently take up histamine, suggesting a role in regulating its plasma levels. This amine uptake mechanism is concentration and time-dependent, exhibiting saturation kinetics.
Area of Science:
- Immunology
- Pharmacology
- Cell Biology
Background:
- Histamine is a key mediator in allergic and inflammatory responses.
- Understanding histamine's fate in circulation is crucial for managing related conditions.
Purpose of the Study:
- To investigate the capacity of normal human neutrophils to uptake histamine.
- To determine the kinetic parameters of histamine uptake by neutrophils.
Main Methods:
- Incubation of human neutrophils with varying concentrations of radiolabeled histamine ((2.5-3H)histamine).
- Measurement of histamine uptake at different time points (20, 60, 90 minutes) at 37.5°C.
- Analysis of uptake data in relation to histamine concentration and incubation time.
Main Results:
- Neutrophil histamine uptake demonstrated a clear correlation with both amine concentration and incubation duration.
- The uptake process exhibited saturation kinetics, indicating a finite transport capacity.
- A significant capacity for histamine accumulation by neutrophils was observed.
Conclusions:
- Neutrophils possess a mechanism for actively taking up histamine from the surrounding environment.
- These findings suggest that neutrophils may play a significant role in the physiological regulation of plasma histamine levels.
- This cellular uptake mechanism could influence histamine bioavailability and its downstream effects.
