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Updated: May 21, 2026

Applying Advanced In Vitro Culturing Technology to Study the Human Gut Microbiota
Published on: February 15, 2019
In vivo veritas, in vitro artificia
Giuseppe Matarese1, Antonio La Cava, Tamas L Horvath
1Dipartimento di Medicina e Chirurgia, Università degli Studi di Salerno, Baronissi Campus, Salerno 84081, Italy. gmatarese@unisa.it
Abstract:
Recently, the understanding of dynamic cellular changes that occur in vivo has advanced significantly, both at the extracellular and intracellular levels. These changes might fluctuate with daily, circadian, weekly, or monthly intervals, and the approaches used to understand these changing conditions in vitro should parallel in vivo studies. In addition, the in vitro milieu should be optimized and better defined, so that artifacts due to in vitro culture systems would not pose dangers for the proper interpretation of results. In this article, we discuss some of these issues and propose solutions.
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