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Nucleosome unit repeat size in aneuploid mice.

A V Rake1

  • 1Biological Sciences, Wright State University, Dayton, Ohio 45435.

Biochemical Genetics
|August 1, 1990
PubMed
Summary

A specific chromosomal abnormality in male mice, trisomy for a segment of chromosome 7, leads to a smaller nucleosome repeat unit size. This finding was consistent in both young and adult mice.

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Area of Science:

  • Genetics
  • Molecular Biology
  • Epigenetics

Background:

  • Nucleosome repeat unit size is a fundamental characteristic of chromatin structure.
  • Chromosomal abnormalities can impact gene expression and cellular processes.
  • Previous research suggested a link between genetic makeup and chromatin organization.

Purpose of the Study:

  • To investigate the effect of specific chromosomal aneuploidies on nucleosome repeat unit size in mice.
  • To determine if a particular chromosomal translocation and resulting trisomy impacts chromatin structure.
  • To compare nucleosome size in genetically altered mice with their normal littermates.

Main Methods:

  • Comparison of nucleosome repeat unit size in male mice with a specific chromosomal translocation [T(X;7)1 Ct] and trisomy for a segment of chromosome 7 versus normal littermates.
  • Assessment of nucleosome size in both young (8-day-old) and adult trisomic male mice.
  • Evaluation of nucleosome unit size in other aneuploid mouse models (XO vs XX and Ts12(17) vs normal).

Main Results:

  • Male mice with trisomy for a segment of chromosome 7 exhibited a smaller nucleosome repeat unit size compared to normal littermates.
  • This reduction in nucleosome size was observed in both juvenile and adult trisomic males.
  • Males with a balanced chromosomal translocation [T(X;7)1Ct] showed normal nucleosome size.
  • Nucleosome unit size remained unaltered in XO vs XX and Ts12(17) vs normal aneuploid mice.

Conclusions:

  • A specific chromosomal imbalance, trisomy for a portion of chromosome 7, directly influences chromatin structure by reducing nucleosome repeat unit size.
  • The observed change in nucleosome size is a stable characteristic associated with this particular aneuploidy.
  • Other forms of aneuploidy, such as sex chromosome aneuploidy or different autosomal trisomies, do not appear to affect nucleosome repeat unit size.

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