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Crystallization and preliminary crystallographic analysis of D-aspartate oxidase from porcine kidney
Miki Senda1, Atsushi Yamamoto, Hiroyuki Tanaka
1Structure Guided Drug Development Project, JBIC Research Institute, Japan Biological Informatics Consortium, 2-4-7 Aomi, Koto-ku, Tokyo 135-0064, Japan. toshiya-senda@aist.go.jp
Abstract:
D-Aspartate oxidase (DDO) from porcine kidney was crystallized by the sitting-drop vapour-diffusion method using PEG 8000 as a precipitant. The crystal belonged to space group P2(1), with unit-cell parameters a = 79.38, b = 144.0, c = 80.46 Å, β = 101.1°, and diffracted to 1.80 Å resolution. Molecular-replacement trials using the structure of human D-amino-acid oxidase, which is 42% identical in sequence to DDO, as a search model provided a satisfactory solution.
