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Application of DNA Barcoding to Identify Medicinal Plants
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DNA barcoding birds: from field collection to data analysis.

Darío A Lijtmaer1, Kevin C R Kerr, Mark Y Stoeckle

  • 1Ornithology, "Bernardino Rivadavia", Buenos Aires, Argentina. dariolijtmaer@gmail.com

Methods in Molecular Biology (Clifton, N.J.)
|June 12, 2012
PubMed
Summary
This summary is machine-generated.

This study details the methodology for DNA barcoding birds, using a specific gene segment. It covers specimen collection, DNA extraction, PCR, sequencing, and data analysis for avian species identification.

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Area of Science:

  • Genomics
  • Ornithology
  • Molecular Biology

Background:

  • DNA barcoding is crucial for species identification.
  • Over 23,000 avian specimens from 3,800 species have been barcoded.
  • A standardized methodology for avian DNA barcoding is needed.

Purpose of the Study:

  • To detail the methodology for obtaining DNA barcodes from birds.
  • To highlight key aspects relevant to avian DNA barcoding.
  • To provide a comprehensive guide from specimen collection to data analysis.

Main Methods:

  • Collection of avian specimens.
  • DNA extraction and Polymerase Chain Reaction (PCR).
  • Sequencing of the COI gene and data analysis.

Main Results:

  • Successful amplification and sequencing protocols for diverse avian species.
  • Methodology covers specimen handling, DNA extraction, PCR, sequencing, and data analysis.
  • Detailed primer pairs and thermal cycling profiles are provided.

Conclusions:

  • The described methodology facilitates robust avian DNA barcoding.
  • This approach aids in species identification, error detection, and lineage analysis.
  • The study contributes to a comprehensive avian DNA barcode library.