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Updated: May 21, 2026

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells

Published on: January 7, 2020

DNA barcoding in mammals.

Natalia V Ivanova¹, Elizabeth L Clare, Alex V Borisenko

¹Biodiversity Institute of Ontario & Integrative Biology, University of Guelph, Guelph, ON, Canada.

Methods in Molecular Biology (Clifton, N.J.)

|June 12, 2012

Summary

This summary is machine-generated.

DNA barcoding aids mammal identification and discovery of new species. This study details three methods for genetic analysis, applicable to various vertebrates, from routine library building to forensic applications.

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Area of Science:

Molecular Biology
Genetics
Zoology

Background:

DNA barcoding is crucial for mammalian taxonomy and discovering cryptic species.
A substantial reference library of over 35,000 mammalian cytochrome c oxidase subunit I sequences has been established.
This effort defines the scope of mammal-focused DNA barcoding initiatives.

Purpose of the Study:

To outline methodological pathways for mammalian DNA barcoding.
To present routine, express, and forensic techniques for genetic identification.
To highlight the broad applicability of these methods beyond Mammalia.

Main Methods:

Routine methods for building reference sequence libraries from high-quality samples.
Express approaches for rapid and cost-effective taxonomic identification in applied settings.
Forensic techniques tailored for analyzing degraded biological material.

Main Results:

Successful assembly of a large mammalian DNA barcode reference library.
Development of distinct methodological approaches for different barcoding needs.
Demonstration of the versatility of described methods across vertebrate taxa.

Conclusions:

Mammalian DNA barcoding offers a robust framework for species identification and discovery.
A tiered approach using routine, express, and forensic methods optimizes genetic analysis.
The described techniques are transferable to a wide array of vertebrate species.