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Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
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Related Experiment Videos

ProMEX - a mass spectral reference database for plant proteomics.

Stefanie Wienkoop1, Christiana Staudinger, Wolfgang Hoehenwarter

  • 1Department of Molecular Systems Biology, University of Vienna, Vienna, Austria.

Frontiers in Plant Science
|June 12, 2012
PubMed
Summary

The ProMEX database offers annotated tryptic peptides for plant proteomics research. It provides experimental MS/MS data for specific proteomes, aiding in plant science discovery.

Keywords:
bioinformaticsfunctional databasesmass spectrometryplant biologyproteomicssystem biology

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Area of Science:

  • Plant proteomics
  • Bioinformatics
  • Molecular biology

Background:

  • Annotated tryptic peptides are crucial for understanding plant proteomes.
  • Existing databases may lack comprehensive cell type-specific or sub-cellular proteomic data.

Purpose of the Study:

  • To establish ProMEX as a primary resource for annotated tryptic peptides in plant proteomics.
  • To provide experimental MS/MS-based proteomic information for various plant and microbial species.

Main Methods:

  • Curating and annotating tryptic peptide data.
  • Integrating MS/MS spectral information.
  • Linking protein data to external databases and pathway maps.

Main Results:

  • ProMEX contains annotated tryptic peptides for multiple plant and microbial species.
  • Experimental MS/MS data is available for cell type-specific or sub-cellular proteomes.
  • Direct links to relevant protein databases and pathway visualizations are provided.

Conclusions:

  • ProMEX serves as a valuable, centralized resource for plant proteomics research.
  • The database facilitates deeper investigation into plant cellular and sub-cellular proteomes.
  • Enhanced data accessibility through external links and pathway maps supports broader scientific inquiry.