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Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or quantified.

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Related Experiment Video

Updated: May 21, 2026

Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus

Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus

Published on: March 8, 2012

[ELISA test-kit for herpes 2 serodiagnosis].

H V Kovtoniuk, L M Korshun, V O Shevchuk

Mikrobiolohichnyi Zhurnal (Kiev, Ukraine : 1993)

|June 13, 2012

Summary

A new ELISA test kit using recombinant glycoprotein gG2 can detect Herpes simplex virus 2 specific IgG and its avidity. This tool aids in diagnosing primary infections, chronic disease, and reactivation, improving herpetic infection management.

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Last Updated: May 21, 2026

Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus

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Published on: March 8, 2012

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Published on: May 11, 2020

Development and Validation of a Quantitative PCR Method for Equid Herpesvirus-2 Diagnostics in Respiratory Fluids

Development and Validation of a Quantitative PCR Method for Equid Herpesvirus-2 Diagnostics in Respiratory Fluids

Published on: March 17, 2016

Area of Science:

Immunology
Virology
Medical Diagnostics

Background:

Herpes simplex virus 2 (HSV-2) infections pose diagnostic challenges.
Differentiating primary infection from chronic disease or reactivation is crucial for effective management.
Assessing antibody avidity can provide insights into the stage of infection.

Purpose of the Study:

To develop and validate an Enzyme-Linked Immunosorbent Assay (ELISA) test kit for detecting HSV-2 specific IgG antibodies.
To determine the avidity of these antibodies using the developed test kit.
To evaluate the utility of the ELISA test kit in diagnosing different stages of HSV-2 infection.

Main Methods:

Construction of an ELISA test kit utilizing recombinant HSV-2 glycoprotein gG2 (PSC SPC Diaproph-Med).
Investigation of the distribution of specific IgG antibodies with varying avidity indexes in healthy donor samples.
Application of the test kit for analyzing antibody profiles in different clinical scenarios.

Main Results:

The developed ELISA test kit successfully detected Herpes simplex virus 2 specific IgG.
The kit allowed for the determination of antibody avidity indexes.
Analysis revealed the distribution of different avidity levels in the tested population.

Conclusions:

The developed ELISA test kit is a viable tool for detecting HSV-2 specific IgG and assessing antibody avidity.
This test kit can aid in confirming primary HSV-2 infections.
The kit shows potential for differentiating between primary infection, chronic disease, and viral reactivation, serving as an additional diagnostic aid for herpetic infections.