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Related Concept Videos

Assembly of Signaling Complexes01:30

Assembly of Signaling Complexes

Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
Intracellular Signaling Cascades01:24

Intracellular Signaling Cascades

Once a ligand binds to a receptor, the signal is transmitted through the membrane and into the cytoplasm. The continuation of a signal in this manner is called signal transduction. Signal transduction only occurs with cell-surface receptors, which cannot interact with most components of the cell, such as DNA. Only internal receptors can interact directly with DNA in the nucleus to initiate protein synthesis. When a ligand binds to its receptor, conformational changes occur that affect the...
Intracellular Signaling Cascades01:24

Intracellular Signaling Cascades

Once a ligand binds to a receptor, the signal is transmitted through the membrane and into the cytoplasm. The continuation of a signal in this manner is called signal transduction. Signal transduction only occurs with cell-surface receptors, which cannot interact with most components of the cell, such as DNA. Only internal receptors can interact directly with DNA in the nucleus to initiate protein synthesis. When a ligand binds to its receptor, conformational changes occur that affect the...
IP3/DAG Signaling Pathway01:11

IP3/DAG Signaling Pathway

Membrane lipids such as phosphatidylinositol (PI) are precursors for several membrane-bound and soluble second messengers. Specific kinases phosphorylate PI and produce phosphorylated inositol phospholipids. One such inositol phospholipids are the  phosphatidylinositol-4,5 bisphosphate [PI(4,5)P2], present in the inner half of the lipid bilayer. Upon ligand binding, GPCR stimulates Gq proteins to turn on phospholipase Cꞵ. Activated phospholipase Cꞵ cleaves PI(4,5)P2 and produces two-second...
Amplifying Signals via Enzymatic Cascade01:22

Amplifying Signals via Enzymatic Cascade

When a ligand binds to a cell-surface receptor, the receptor's intracellular domain changes shape, which may either activate its enzyme function or allow its binding to other molecules. The initial signal is amplified by most signal transduction pathways. This means that a single ligand molecule can activate multiple molecules of a downstream target. Proteins that relay a signal are most commonly phosphorylated at one or more sites, activating or inactivating the protein. Kinases catalyze the...
Calmodulin-dependent Signaling01:16

Calmodulin-dependent Signaling

Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
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Related Experiment Video

Updated: May 21, 2026

Imaging G-protein Coupled Receptor (GPCR)-mediated Signaling Events that Control Chemotaxis of Dictyostelium Discoideum
09:40

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Cyclic nucleotide phosphodiesterase 3 signaling complexes.

F Ahmad1, E Degerman, V C Manganiello

  • 1Cardiovascular Pulmonary Branch, National Heart, Lung and Blood Institute, Bethesda, MD 20892, USA. ahmadF@NHLBI.NIH.GOV

Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Metabolisme
|June 14, 2012
PubMed
Summary

Cyclic nucleotide phosphodiesterases (PDEs) regulate cellular signaling. This study identifies PDE3A and PDE3B signalosomes, revealing their roles in specific cellular compartments and cAMP-mediated metabolic processes.

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Area of Science:

  • Biochemistry
  • Cell Biology
  • Molecular Pharmacology

Background:

  • Cyclic nucleotide phosphodiesterases (PDEs) are crucial enzymes regulating intracellular cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP) levels.
  • PDEs control cyclic nucleotide-dependent signaling pathways, impacting numerous physiological processes.
  • The PDE3 subfamily, comprising PDE3A and PDE3B, plays significant roles in cellular regulation.

Purpose of the Study:

  • To highlight the identification of distinct PDE3A- and PDE3B-containing signalosomes.
  • To elucidate the role of these signalosomes in specialized subcellular compartments.
  • To understand their involvement in regulating cAMP-mediated metabolic processes.

Main Methods:

  • Analysis of PDE3A and PDE3B expression patterns in various cell types.
  • Investigation of PDE-containing macromolecular signaling complexes (signalosomes).
  • Characterization of subcellular localization and functional impact of PDE signalosomes.

Main Results:

  • PDE3B is predominantly expressed in metabolic regulatory cells (adipocytes, hepatocytes, pancreatic β-cells).
  • PDE3A shows higher expression in cardiac, vascular, and reproductive cells.
  • Identification of specific PDE3A and PDE3B signalosomes in distinct subcellular compartments.

Conclusions:

  • PDE3A and PDE3B signalosomes enhance specificity and efficiency of intracellular signaling.
  • These signalosomes are involved in the regulation of diverse cAMP-mediated metabolic processes.
  • Understanding PDE signalosome localization and function is key to deciphering cellular signaling.