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Updated: May 21, 2026

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Self-calibrating viscosity probes: design and subcellular localization.

Marianna Dakanali1, Thai H Do, Austin Horn

  • 1Department of Chemistry & Biochemistry, University of California, San Diego, 9500 Gilman Drive, MC: 0358, La Jolla, CA 92093-0358, USA.

Bioorganic & Medicinal Chemistry
|June 16, 2012
PubMed
Summary
This summary is machine-generated.

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Researchers developed self-calibrating viscosity dyes by linking coumarin and molecular rotors. These probes enable accurate viscosity measurements within cellular environments, with one dye suitable for cytoplasm analysis.

Area of Science:

  • Chemical Biology
  • Biophysical Chemistry

Background:

  • Viscosity is a critical biophysical parameter influencing cellular processes.
  • Developing precise intracellular viscosity probes remains a challenge.

Purpose of the Study:

  • To design and synthesize novel self-calibrating viscosity dyes.
  • To characterize their fluorescence properties and subcellular localization.
  • To develop probes for accurate intracellular viscosity measurements.

Main Methods:

  • Covalent linkage of coumarin (reference fluorophore) with molecular rotors (viscosity sensors).
  • Characterization of fluorescence properties via separate excitation and resonance energy transfer.
  • Modification of linker and rotor substitution to tune hydrophilicity and localization.

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Published on: November 23, 2015

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Last Updated: May 21, 2026

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05:49

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Published on: December 2, 2022

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions
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A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

Published on: November 23, 2015

Main Results:

  • Successful synthesis and characterization of self-calibrating viscosity dyes.
  • Demonstrated ability to measure viscosity through fluorescence changes.
  • Hydrophilic dye 12 exhibits homogeneous cellular distribution, suitable for cytoplasmic viscosity mapping.

Conclusions:

  • The developed dyes offer a self-calibrating approach for viscosity sensing.
  • Modulating probe hydrophilicity allows for targeted subcellular localization.
  • Hydrophilic dye 12 is a promising tool for real-time cytoplasmic viscosity monitoring.