Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Glycosylation01:25

Protein Glycosylation

Glycosylation, the most common post-translational modification for proteins, serves diverse functions. Adding sugars to proteins makes the proteins more resistant to proteolytic digestion. Glycosylated proteins can act as markers and receptors to promote cell-cell adhesion. Additionally, they have many essential quality control functions in the cell, such as correct protein folding and facilitating transport of misfolded proteins to the cytosol, which can be degraded.
Glycosylation occurs in...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The impact of alcohol consumption on bone mineral density: Insights from cross-sectional and Mendelian randomization studies.

Bone·2026
Same author

Flexible infrared camouflage eutectic gallium-indium for thermoelectric energy harvesting.

Nature communications·2026
Same author

Oat-β-glucan potentiates anti-PD-1 efficacy through Faecalibacterium prausnitzii-derived butyrate and indole-3-propionic acid.

Cell host & microbe·2026
Same author

Closed-loop tactile-visual interactivity via chip-free luminescent fibers enabled by capacitive coupling.

Science advances·2026
Same author

Associations of NMR metabolic biomarkers and arterial calcification: An observational and Mendelian randomization study within the BBMRI metabolomics consortium.

Atherosclerosis plus·2026
Same author

Gemella morbillorum Promotes Colorectal Carcinogenesis: LPBDCP-Mediated Invasion Activates Ras Signaling and Destabilizes p53.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same journal

Homo-oligomeric forms of lectins: their structural insights for glycan binding from the genus Canavalia.

Glycoconjugate journal·2026
Same journal

Correction to: Alpha-Gal syndrome unveiled: decoding the glycan-driven red meat allergy.

Glycoconjugate journal·2026
Same journal

Mechanistic and functional integration of sirtuin deacylases: from substrate specificity to biological outcomes.

Glycoconjugate journal·2026
Same journal

Macrophage modulation accounts for the anti-inflammatory effect of Hypnea cervicornis agglutinin in rat arthritis induced by zymosan.

Glycoconjugate journal·2026
Same journal

Nucleolar-localized sialidase Neu4 suppresses cell proliferation through destabilizing c-Myc via interacting with ribosomal proteins L5 and L11.

Glycoconjugate journal·2026
Same journal

3'-sialyllactose inhibits the migration and invasion of LPS-induced B16F10 mouse melanoma cells through inactivation of the NF-<sub>K</sub>B signaling pathway.

Glycoconjugate journal·2026
See all related articles

Related Experiment Video

Updated: May 18, 2026

A Quantitative Glycomics and Proteomics Combined Purification Strategy
11:38

A Quantitative Glycomics and Proteomics Combined Purification Strategy

Published on: March 8, 2016

Recent progress in quantitative glycoproteomics.

Ying Zhang1, Hongrui Yin, Haojie Lu

  • 1Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai, China.

Glycoconjugate Journal
|June 16, 2012
PubMed
Summary
This summary is machine-generated.

Quantitative glycoproteomics analyzes protein glycosylation changes linked to diseases. This review summarizes mass spectrometry techniques for N-linked and O-linked glycoproteome analysis, aiding biomarker discovery.

More Related Videos

Rapid Glyco-Qualitative Assessment of Recombinant Proteins Using a Fully Automated System
05:19

Rapid Glyco-Qualitative Assessment of Recombinant Proteins Using a Fully Automated System

Published on: June 28, 2024

Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments
10:59

Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments

Published on: February 7, 2025

Related Experiment Videos

Last Updated: May 18, 2026

A Quantitative Glycomics and Proteomics Combined Purification Strategy
11:38

A Quantitative Glycomics and Proteomics Combined Purification Strategy

Published on: March 8, 2016

Rapid Glyco-Qualitative Assessment of Recombinant Proteins Using a Fully Automated System
05:19

Rapid Glyco-Qualitative Assessment of Recombinant Proteins Using a Fully Automated System

Published on: June 28, 2024

Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments
10:59

Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments

Published on: February 7, 2025

Area of Science:

  • Biochemistry and Molecular Biology
  • Proteomics
  • Glycoscience

Background:

  • Protein glycosylation is a critical posttranslational modification impacting protein function and cellular processes.
  • Altered glycosylation patterns are hallmarks of various diseases, highlighting their diagnostic potential.
  • Quantitative glycoproteomics offers powerful tools for biomarker discovery and understanding disease mechanisms.

Purpose of the Study:

  • To review and summarize recent advancements in quantitative glycoproteomics technologies.
  • To focus on mass spectrometry-based approaches for N-linked and O-linked glycosylation analysis.
  • To discuss the strengths and limitations of current quantitative glycoproteomic methodologies.

Main Methods:

  • Mass spectrometry-based protein quantification techniques.
  • Glycoprotein and glycopeptide enrichment strategies.
  • Analysis of N-linked and O-linked glycoproteomes.

Main Results:

  • Recent technologies enable systematic and quantitative assessment of glycoproteome differences.
  • Combined enrichment and analysis methods enhance quantitative glycoproteomics applications.
  • Despite progress, comprehensive quantitative analysis of complex biological samples remains challenging.

Conclusions:

  • Quantitative glycoproteomics is a rapidly advancing field crucial for biomarker discovery.
  • Understanding the strengths and weaknesses of various techniques is essential for effective application.
  • Further development of universal quantitative technologies is needed for complex glycoproteome analysis.