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Related Concept Videos

Microtubule Instability02:17

Microtubule Instability

Microtubules are hollow cylindrical filaments having a diameter of approximately 25 nm and a length that varies from 200 nm to 25 μm. GTP-bound tubulin subunits form αβ-heterodimers for microtubule assembly. These core building blocks interact longitudinally, polymerizing into protofilaments. The protofilaments then interact with one another through lateral bonding forces to form stable cylindrical microtubules. These cylindrical filaments are dynamic as they undergo repeated assembly and...
Microtubule Instability02:17

Microtubule Instability

Microtubules are hollow cylindrical filaments having a diameter of approximately 25 nm and a length that varies from 200 nm to 25 μm. GTP-bound tubulin subunits form αβ-heterodimers for microtubule assembly. These core building blocks interact longitudinally, polymerizing into protofilaments. The protofilaments then interact with one another through lateral bonding forces to form stable cylindrical microtubules. These cylindrical filaments are dynamic as they undergo repeated assembly and...
Centrosome Duplication02:25

Centrosome Duplication

The primary microtubule organizing center (MTOC) in animal cells is the centrosome. A centrosome has two cylindrical centrioles at its core. Each centriole consists of nine sets of three microtubules held together by proteins. The centrioles are positioned at right angles to each other and surrounded by a shapeless protein cloud called the pericentriolar matrix, or pericentriolar material (PCM).
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...
Centrosome Duplication02:25

Centrosome Duplication

The primary microtubule organizing center (MTOC) in animal cells is the centrosome. A centrosome has two cylindrical centrioles at its core. Each centriole consists of nine sets of three microtubules held together by proteins. The centrioles are positioned at right angles to each other and surrounded by a shapeless protein cloud called the pericentriolar matrix, or pericentriolar material (PCM).
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...
Separation of Sister Chromatids02:17

Separation of Sister Chromatids

At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
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Centrioles and Centrosomes01:13

Centrioles and Centrosomes

Most animal cells comprise a pair of centrioles together called a centrosome. The cell duplicates its centrosome and contains two centrosomes side-by-side, which begin to move apart during the prophase. As the centrosomes migrate to two different sides of the cell, microtubules start extending from each centrosome toward the other end. The mitotic spindle is composed of the centrosomes and their emerging microtubules.
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Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes
09:39

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Published on: December 20, 2014

Tankyrase 1 regulates centrosome function by controlling CPAP stability.

Mi Kyung Kim1, Charles Dudognon, Susan Smith

  • 1Molecular Pathogenesis Program and Department of Pathology, Kimmel Center for Biology and Medicine of the Skirball Institute, New York University School of Medicine, New York, NY 10016, USA.

EMBO Reports
|June 16, 2012
PubMed
Summary

Tankyrase 1 controls CPAP protein levels via PARsylation, regulating centriole duplication. This ensures normal centrosome function by limiting centriole elongation during the cell cycle.

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Last Updated: May 21, 2026

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes
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Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • Centriole duplication is essential for cell division and centrosome function.
  • CPAP (centrosome-associated protein) is a crucial gene for procentriole formation, and its mutation causes primary microcephaly.
  • The regulation of CPAP levels and function during the cell cycle remains incompletely understood.

Purpose of the Study:

  • To investigate the role of tankyrase 1 in regulating CPAP protein stability and function.
  • To elucidate the mechanism by which tankyrase 1 controls CPAP during the cell cycle.
  • To understand how this regulation impacts centriole duplication and centrosome integrity.

Main Methods:

  • In vitro and in vivo poly(ADP-ribose)ylation assays to assess CPAP modification by tankyrase 1.
  • Overexpression and depletion studies of tankyrase 1 in cell culture.
  • Cell cycle analysis and centrosome morphology assessment using microscopy.

Main Results:

  • Tankyrase 1 directly PARsylates CPAP, targeting it for proteasomal degradation.
  • Tankyrase 1 overexpression leads to CPAP degradation, inhibiting centriole duplication.
  • Tankyrase 1 depletion stabilizes CPAP in G1 phase, resulting in elongated procentrioles and centrosome multipolarity.

Conclusions:

  • Tankyrase 1 acts as a key regulator of CPAP protein levels through PARsylation-mediated degradation.
  • Tankyrase 1 localization to centrosomes in G1 phase coordinates CPAP degradation with the cell cycle.
  • This regulatory mechanism is critical for limiting centriole elongation and ensuring proper centrosome duplication and function.